Phytochemical and Antioxidant Properties of Athamanta turbith (L.) Brot Collected from Serbia
2021
Аутори:
Kilibarda, SofijaVuković, Sandra
Milinčić, Danijel
Mačukanović-Jocić, Marina
Jarić, Snežana
Kostić, Aleksandar
Остала ауторства
Samuoliene, GiedreТип документа:
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Athamanta turbith (L.) Brot. is the endemic flowering plant from the Apiaceae family
originated from Đetinja Canyon (Serbia). The aim of this study was to determine the content of
selected plant bioactive compounds present in rhizome, vegetative shoot and inflorescence. Extraction
was performed in 80% methanol as a solvent with two different approaches: powdered
plant material was extracted with solvent for 3 h in ratio 1:10 without (classical solvent extraction,
SE,) and with application of ultrasound (ultrasound-assisted extraction, UAE). Analysis of total
phenolic content (TPC), total flavonoid content (TFC) and total hydroxycinnamic acid derivative
content (HCA) was performed via spectrophotometric methods. The inflorescence had the highest
TPC in UAE obtained-extract (2.73 ± 0.13 mg GAE/g), as well as the highest TFC (1.56 ± 0.02 mg/g
QE) and HCA (1.45 ± 0.11 mg/g CGAE) in SE-prepared extract where GAE, QE and CGAE are gallic
acid equivalents, quercetin equivalents and chlorogenic acid equivalents respectively. The lowest
amount of TPC, TFC and HCA was detected in the rhizome regardless of extraction methods. Additionally,
antioxidant properties of extracts were determined with five assays: ABTS∙+, DPPH∙,
ferric reducing power (FRP), in vitro phosphomolybdenum total antioxidant capacity (TAC) and
cupric reducing antioxidant capacity (CUPRAC). The inflorescence had the highest antioxidant
activity in both quencher assays (at γ = 0.1 g/mL) with 92.1% of inhibition for ABTS∙+ (UAE extract,)
and 77.7% inhibition of DPPH∙ (for both extracts). In addition, it exhibited the highest FRP (18.4
mg/g AAE, SE extract and CUPRAC (~40 mg/g AAE for both extracts) values where AAE stands
for ascorbic acid equivalents. Whereas, the rhizome had the lowest values for all antioxidant assays
concerning both SE and UEA. In conclusion, exhibited antioxidant properties are mostly in line
with the determined content of selected bioactive compounds. Further statistical analysis is applied
to confirm/oppose this.
Кључне речи:
Athamanta turbith; bioactive compounds; antioxidant activity; Đetinja CanyonФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200116 (Универзитет у Београду, Пољопривредни факултет) (RS-MESTD-inst-2020-200116)
У:
- Samuoliene G, editor. The 2nd International Electronic Conference on Plant Sciences—10th Anniversary of Journal Plants; 2021 Dec 1-15; Online. Basel: MDPI; 2021. p. 11947. (Biology and Life Sciences Forum)