dc.creator | Mandić, Miloš | |
dc.creator | Misirkić Marjanović, Maja | |
dc.creator | Vučićević, Ljubica | |
dc.creator | Jovanović, Maja | |
dc.creator | Bošnjak, Mihajlo | |
dc.creator | Perović, Vladimir | |
dc.creator | Ristić, Biljana | |
dc.creator | Ćirić, Darko | |
dc.creator | Harhaji Trajković, Ljubica | |
dc.creator | Trajković, Vladimir | |
dc.date.accessioned | 2022-04-28T11:48:54Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2022 | |
dc.identifier.issn | 0024-3205 | |
dc.identifier.uri | https://linkinghub.elsevier.com/retrieve/pii/S0024320522001813 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/35304128 | |
dc.identifier.uri | http://radar.ibiss.bg.ac.rs/handle/123456789/4947 | |
dc.description.abstract | We investigated the mechanisms and the role of autophagy in the differentiation of HL-60 human acute myeloid leukemia cells induced by protein kinase C (PKC) activator phorbol myristate acetate (PMA). PMA-triggered differentiation of HL-60 cells into macrophage-like cells was confirmed by cell-cycle arrest accompanied by elevated expression of macrophage markers CD11b, CD13, CD14, CD45, EGR1, CSF1R, and IL-8. The induction of autophagy was demonstrated by the increase in intracellular acidification, accumulation/punctuation of autophagosome marker LC3-II, and the increase in autophagic flux. PMA also increased nuclear translocation of autophagy transcription factors TFEB, FOXO1, and FOXO3, as well as the expression of several autophagy-related (ATG) genes in HL-60 cells. PMA failed to activate autophagy inducer AMP-activated protein kinase (AMPK) and inhibit autophagy suppressor mechanistic target of rapamycin complex 1 (mTORC1). On the other hand, it readily stimulated the phosphorylation of mitogen-activated protein (MAP) kinases extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) via a protein kinase C-dependent mechanism. Pharmacological or genetic inhibition of ERK or JNK suppressed PMA-triggered nuclear translocation of TFEB and FOXO1/3, ATG expression, dissociation of pro-autophagic beclin-1 from its inhibitor BCL2, autophagy induction, and differentiation of HL-60 cells into macrophage-like cells. Pharmacological or genetic inhibition of autophagy also blocked PMA-induced macrophage differentiation of HL-60 cells. Therefore, MAP kinases ERK and JNK control PMA-induced macrophage differentiation of HL-60 leukemia cells through AMPK/mTORC1-independent, TFEB/FOXO-mediated transcriptional and beclin-1-dependent post-translational activation of autophagy. | |
dc.publisher | Elsevier Inc. | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200110/RS// | |
dc.relation | COST Action CA15138 | |
dc.rights | restrictedAccess | |
dc.source | Life Sciences | |
dc.subject | Autophagy | |
dc.subject | Beclin-1 | |
dc.subject | Differentiation | |
dc.subject | ERK | |
dc.subject | JNK | |
dc.subject | Leukemia | |
dc.title | MAP kinase-dependent autophagy controls phorbol myristate acetate-induced macrophage differentiation of HL-60 leukemia cells. | |
dc.type | article | en |
dc.rights.license | ARR | |
dc.rights.holder | © 2022 Elsevier Inc. | |
dc.citation.volume | 297 | |
dc.identifier.doi | 10.1016/j.lfs.2022.120481 | |
dc.identifier.pmid | 35304128 | |
dc.identifier.scopus | 2-s2.0-85126891813 | |
dc.identifier.wos | 000793214700005 | |
dc.citation.apa | Mandic, M., Misirkic Marjanovic, M., Vucicevic, L., Jovanovic, M., Bosnjak, M., Perovic, V., et al. (2022). MAP kinase-dependent autophagy controls phorbol myristate acetate-induced macrophage differentiation of HL-60 leukemia cells. Life Sciences, 297, 120481. | |
dc.citation.vancouver | Mandic M, Misirkic Marjanovic M, Vucicevic L, Jovanovic M, Bosnjak M, Perovic V, Ristic B, Ciric D, Harhaji-Trajkovic L, Trajkovic V. MAP kinase-dependent autophagy controls phorbol myristate acetate-induced macrophage differentiation of HL-60 leukemia cells. Life Sci. 2022;297:120481. | |
dc.citation.spage | 120481 | |
dc.type.version | publishedVersion | |
dc.citation.rank | M21~ | |