Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats
2022
Аутори:
Filipović, BrankoAjdžanović, Vladimir
Živanović, Jasmina
Trifunović, Svetlana
Ristić, Nataša
Milošević, Verica
Šošić-Jurjević, Branka
Тип документа:
Чланак у часопису (Објављена верзија)
,
© The Author(s), 2022. Published by Cambridge University Press on behalf of the Microscopy Society of America
Метаподаци
Приказ свих података о документуАпстракт:
Thyroid C-cells secrete the hormone calcitonin (CT) which acts as an inhibitor of bone resorption. Our aim was to examine the age-related changes in the structure and function of CT-producing C-cells, using histomorphometric, ultrastructural, and biochemical analyses. We used young adult (3-months-old), middle-aged (16-months-old), and old (24-months-old) male rats. The peroxidase-antiperoxidase method was applied for localization of CT. Stereological analysis was performed using the newCAST stereological software package. Serum samples were analyzed for the determination of CT, testosterone (T), calcium (Ca2+), and phosphorus (P). We found a significant increase in the volume density (Vv) of C-cells in both older groups (p < 0.05). The percentage of smaller volume range C-cells increased (p < 0.0001), while the proportion of greater volume range C-cells decreased (p < 0.05) with ageing. Ultrastructural analysis revealed a larger number of secretory granules in older rats. Serum CT increased (p < 0.001), while serum T and P were reduced (p < 0.01) in older rats. Serum Ca2+ was lower (p < 0.0001) in middle-aged rats compared to young adults. We revealed a 20% incidence of C-cell hyperplasia in older rats and one case of medullary thyroid carcinoma in an old rat. Our findings indicate that the ageing process causes significant histomorphometric changes at the thyroid C-cell level.
Кључне речи:
ageing; calcitonin; histomorphometric analysis; rats; thyroid C-cellsИзвор:
Microscopy and Microanalysis, 2022Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
DOI: 10.1017/S1431927622000721
ISSN: 1431-9276
PubMed: 35592886