Allelopathic effect of apple hairy root exudates on the germination and seedlings growth of Arabidopsis thaliana in vitro
2018
Аутори:
Stanišić, MarianaĆosić, Tatjana
Savić, Jelena
Smigocki, Ann
Ninković, Slavica
Banjac, Nevena
Остала ауторства
Uzelac, BrankaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2018 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
In the previous study, phenolic acids and polyphenolic flavonoids were identified as putative
allelochemicals in apple cv. Golden Delicious hairy roots growth medium. In order to examine
their allelopathic potential, the effect of hairy root exudates on the germination and seedling
growth of the test species Arabidopsis thaliana was elucidated. Arabidopsis seeds were cultivated
in Erlenmeyer flasks containing 25 mL of liquid medium in which apple hairy roots were cultivated
for 4 weeks. The effect of apple hairy root growth medium on Arabidopsis was evaluated after 5
and 10 days of seed cultivation. Apple hairy root exudates had no significant effect on Arabidopsis
germination rate, while growth characteristics of seedlings were significantly affected both after
5 and 10 days of treatment. The roots of treated seedlings were significantly shorter (35.1%), with
lower number (74% decrement) of lateral roots compared to control after10 days of treatment.
The growth and morphology of Arabidopsis shoots and leaves have been affected too. Thus, the
shoots of treated seedlings were almost twice shorter compared to control after 5 days of treatment. At the 10th day, this distinction became more pronounced, when two fully expanded leaves
were visible in treated seedlings, while control had completely developed rosettes with six leaves.
In addition, leaves of treated seedlings were smaller and pale in colour compared to control. qPCR
expression analysis of genes involved in regulation of the cell cycle showed reduction of transcript
levels in roots of treated seedlings, with the highest expression decrease detected for CDKA1 and
CYCB2;4 cyclin.
Кључне речи:
allelopathy; allelochemicals; cell cycle genes; phenolic acids; polyphenolic flavonoidsФинансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)
У:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 55.