Efekti deprenila, rezerpina i njihove kombinacije na aktivnost antioksidativnih enzima u mozgu pacova

Abstract

Antioxidant enzyme activities: superoxide dismutases (CuZn SOD and Mn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST) and glutathione reductase (GR) were determined in different brain regions: striatum, hippocampus, hypothalamus with thalamus, as well as in the rest of the brain of rats treated subchronically with L-deprenyl, after a single dose of reserpine and in animals subchronically treated with L-deprenyl and then with reserpine. Our results show that reserpine (p < 0.02), as well as, the combination of deprenyl + reserpine (p<0.01) induced a significant decrease in CAT activity in the rest of the brain and a significant decrease in GSH-Px activity in the hypothalamus with thalamus (p<0.05). Deprenyl expressed no significant effect on antioxidant enzyme activities in the examined brain regions. In experimental group treated with deprenyl and then with reserpine a significant decrease of CuZn SOD activity (p<0.05) in the hippocampus, Mn SOD activity (p<0.05) in the striatum and an increase of CnZn SOD activity (p<0.01) in the rest of the brain were observed. Our results support the opinion that depletion of neuronal catecholamine pool (due to the treatment with reserpine) has a direct influence on antioxidant enzyme activities, while deprenyl probably exerts its effects through interaction with some trophic factors.

Aktivnost antioksidativnih enzima: superoksid dismutaze (CuZn SOD i Mn SOD), katalaze (CAT), glutation peroksidaze (GSH-Px), glutation-S-trans-feraze (GST) i glutation reduktaze (GR) u strijatumu, hipokampusu, hipotalamusu sa talamusom, kao i ostatku mozga, praćena je u eksperimentima subhroničnog tretiranja pacova L-deprenilom, akutnog rezerpinom i u kombinaciji oba. Naši rezultati pokazuju da rezerpin (r<0.02), kao i kombinacija deprenila i rezeršša (r<0.01) dovode do smanjenja aktivnosti CAT u ostatku mozga i aktivnosti GSH-Px (r< 0.05) u hipotalamusu sa talamusom. Deprenil ne izaziva značajne promene enzimske aktivnosti u ispitivanim regionima mozga. U eksperimentalnoj grupi tretiranoj deprenilom, pa rezerpinom dolazi do značajnog smanjenja aktivnosti CuZn SOD (p< 0.005) hipokampusa, aktivnosti Mn SOD strijatuma (p<0i)5) i povećnja aktivnosti CuZn SOD (r<0.01) ostatka mozga. Ovi rezultati ukazuju da iscriljenje pula kateholamina (usled tretmana rezerpinom) ima direktan uticaj na aktivnost antioksidativnih enzima, dok su za efekte deprenila značajne interakcije sa nekim trofičkim faktorima.