Chokeberry (Aronia melanocarpa) fruit extract abrogates melanoma progression through boosting up IFN-γ-producing cells
2022
Autori:
Mićanović, DragicaStojanović, Ivana D.
Koprivica, Ivan
Pejnović, Nada
Šavikin, Katarina
Ćujić-Nikolić, Nada
Saksida, Tamara
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Chokeberry has exhibited cardioprotective, anti-bacterial, immunomodulating and anti-cancer properties. Chokeberry extract (CE) was tested in the model of melanoma induced by B16 cells inoculation in C57BL/6 mice.
CE treatment that began 7 days before inoculation and continued through the observation period, delayed melanoma appearance and increased infiltration of immune cells in the tumor microenvironment (TME). Levels of TNF, perforin, granzyme B and IL-1β did not differ between the CE-treated and control animals, but the TME of CE-treated mice contained more IFN-γ-producing cells and a lesser frequency of CCR5-expressing MDSC. In vitro, CE displayed no direct cytotoxicity to B16 cells. However, splenocytes isolated from CE-treated animals exerted strong cytotoxic effect on B16 cells in vitro. Neutralization of IFN-γ diminished the observed B16 death, suggesting that this effect was mediated mainly by splenocyte-derived IFN-γ.
In conclusion, pre-treatment with CE stimulated the anti-tumor immune response by enhancing IFN-γ-producing cells to act against melanoma.
Ključne reči:
Aronia melanocarpa; Melanoma; B16 cells; Anti-tumor immune response; NK cells; CD8+ T lymphocyteIzvor:
Journal of Functional Foods, 2022, 95, 105185-Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
DOI: 10.1016/j.jff.2022.105185
ISSN: 1756-4646