Effect of osmotic stress conditions on BvSTI-expressing Lotus corniculatus
2015
Аутори:
Savić, JelenaNinković, Sofija
Cingel, Aleksandar
Ćosić, Tatjana
Raspor, Martin
Banjac, Nevena
Smigocki, Ann C.
Ninković, Slavica
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2015 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Plant proteinase inhibitors (PIs) constitute a large and complex widely distributed group of small proteins
involved in the regulation of the plant protein turn-over required in multiple physiological processes.
Beside the roles regarding development and physiology, PIs are known to act in defense against herbivore
insect pests. In recent years, the new intriguing role of plant PIs in abiotic stress tolerance has been implied.
Inhibitor of serine type proteinases, BvSTI isolated from sugar beet pest resistant genotype was introduced
into Bird’s foot trefoil (Lotus corniculatus L.). To determine the effect of osmotic stress on BvSTI-expressing L.
corniculatus, in vitro-grown shoots were exposed to elevated concentration of sucrose (5%, 7% and 9%). After
15 days of treatment, the growth response of sucrose-treated transformed lines 21, 73 and 109, as well as
of nontransformed (NTC) line was determined by measuring the fresh weight and the length increment. Also
shoots were scored for visible symptoms of osmotic stress-induced injuries by visual inspection. In order to
avoid influence of observed phenotypic differences among lines on parameters analyzed under stress conditions,
all values were presented relative to the controls (shoots grown under the same conditions on regular
3% sucrose) of each line. Additionally, the activities of antioxidant enzymes peroxidases (POD) and catalases
(CAT) were determined spectrophotometrically.
Кључне речи:
plant proteinase inhibitor; sugar; Lotus corniculatusФинансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)
У:
- Uzelac B, editor. Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”; 2015. p. 28.