Phytohormone Profiling of the Potato Plants Exposed to French Marigold Essential Oil
2022
Authors:
Stupar, SofijaMotyka, Vaclav
Dobrev, Petre I.
Ćosić, Tatjana
Devrnja, Nina
Tubić, Ljiljana
Savić, Jelena
Contributors
Milutinović, MilicaDocument Type:
Conference object (Published version)
,
© 2022 by the Serbian Plant Physiology Society
Metadata
Show full item recordAbstract:
French marigold (Tagetes patula L.) is a common companion plant for many vegetable crops
because of its repellent effects on herbivorous insects and other pests. However, little is known
about the effects of its essential oil (EO) volatiles on crops physiology. Given the importance of
phytohormones in integrating signals and regulating vital processes in plants, this study focused
on phytohormone profiling of potato (Solanum tuberosum L.) plants exposed to French marigold
EO for different time periods (4h, 8h, and 12h). Results of LC-MS analysis showed alterations in
accumulation of different phytohormones and/or their metabolites after exposure to EO, with the
most pronounced change observed for stress-related phytohormones: ABA’s metabolite ABA-glucose
ester, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. In most
of the analyzed phytohormone groups similar response pattern was observed – an increase in
levels after short time exposure (4h), followed by a decrease to control values after prolonged exposure
(8h and 12h). In order to present changes in activity of the genes included in metabolism
of phytohormones after 8h long exposure, a detailed analysis of data from a previous microarray
study was performed. These results revealed jasmonic acid biosynthetic pathway as the most affected
one, with the highest number of altered transcripts and with one of the genes from this
pathway (12-oxophytodienoate reductase 1-like) having highest expression rate. In the future, the
data obtained from this comprehensive analysis may help to understand the effects of EO on the
physiology and defense strategy of exposed plants, and subsequently predict plant responses.
Keywords:
potato; French marigold; essential oil; volatiles; phytohormonesFunding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Czech Science Foundation (Grant No. 19-12262S)
- Ministry of Education, Youth and Sports of the Czech Republic from European Regional Development Fund - Project “Centre for Experimental Plant Biology” (No. CZ.02.1.01/0.0/0.0/16_019/0000738)
In:
- Milutinović M, editor. Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 76.