Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila
2018
Аутори:
Hočevar, KatarinaBudečević, Sanja
Manitašević Jovanović, Sanja
Остала ауторства
Uzelac, BrankaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2018 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
The 70kD heat shock proteins (Hsp70s) are highly conserved molecular chaperones that play
essential roles in numerous cellular processes, including the folding of newly synthesized and
stress-denatured proteins, the translocation of proteins across membranes, as well as the degradation of aggregated proteins. Here we examined the variation in the abundance of Hsp70s in
different floral organs of the three distinctive color morphs in Iris pumila. Flowers of twenty seven
genotypes raised in a common-garden and belonging to three colour classes: yellow, dark blue
and dark violet, were collected (9 per each class) for an immunoblot analysis. The content of Hsp70
was quantifed in four floral organs: falls, standards, stamens and style arms. The immunoblot analysis revealed that the expression of Hsp70s was floral organ-specifc. While only one Hsp70 isoform
was expressed in the standards and falls, the stamens and style arms expressed two isoforms. The
relative abundance of Hsp70 varied among differed flower organs as well. The lowest value was
recorded in the standards and the highest in the stamens, whereas its abundance in the falls and
style arms appeared to be intermediate. Such variation pattern was detected exclusively in the
dark violet colour morphs. In the yellow flowers, however, the amount of Hsp70 tended to be lower compared to the dark blue or dark violet colour morphs. This study provides original evidence
for organ-specifc Hsp70 expression in flowers of I. pumila plants, likely due to the differential metabolic activities occurring in the cells of these functionally distinctive floral structures.
Кључне речи:
Hsp70; floral organs; Iris pumila; flower colourФинансирање / пројекти:
- Еволуција у лабораторији и адаптације у природи (RS-MESTD-Basic Research (BR or ON)-173007)
У:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 89-90.
Повезане информације:
- Друга верзија
https://radar.ibiss.bg.ac.rs/handle/123456789/5060