Morpho-physiological and ultrastructural changes of Arabidopsis seedlings treated with phloretin
2022
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Authors:
Smailagić, DijanaMarin, Marija
Banjac, Nevena
Ninković, Slavica
Stanišić, Mariana
Contributors
Milutinović, MilicaDocument Type:
Conference object (Published version)
,
© 2022 by the Serbian Plant Physiology Society
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Show full item recordAbstract:
Phloretin is a phenolic compound unique for apple (Malus × domestica Borkh.) species. Our
research group recently reported phytotoxic potential of phloretin towards other plant species
such as model plant Arabidopsis. Since sugars in in vitro growth media could modulate plant
metabolism, we analyzed phloretin effects on Arabidopsis seedlings grown on the medium with out sucrose and myoinositol (WSM) but enriched with different concentrations of phloretin (0
to 1500 µM). Phloretin treatment on WSM induced seedlings growth retardation at much lower
concentrations compared to sucrose and myoinositol enriched medium. Inhibition of primary
root and leaf growth, and lateral root emergence increased with both phloretin concentration
and treatment duration (maximal 85.2%, 30.4% and 46.5% at 1500 µM phloretin at 15 days after
germination - DAG, respectively). Prolonged cultivation (4 weeks) at phloretin concentrations over
500 µM induced complete growth stagnation and necrosis of the leaves. Transmission electron
microscopy reviled relocation of chloroplasts within mesophyll cells, shape change into spherical
form, as well as ultrastructural changes in thylakoid organization in leaves of 10 DAG-old seedlings
treated with 250 or 500 µM phloretin. Significantly decreased number of starch grains in chloro plasts and chlorophyll a and b content in leaves reflected impaired function of chloroplasts due
to ultrastructural changes. Phloretin also induced cell wall undulation and folds that resulted in
irregularly shaped mesophyll cells. The presented study revealed that beside roots that had been
previously emphasized as primary targets of phloretin action, leaves were also significantly affect ed by phloretin treatment, especially in the aspect of photosynthetic apparatus
Keywords:
bioherbicide; Malus × domestica Borkh.; phloretin; phytotoxicity; ultrastructural changesFunding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
In:
- Milutinović M, editor. Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 72.