Phloretin, dihydrochalcone from apple tree, bursts antioxidant capacity of tested Arabidopsis seedlings
2022
Аутори:
Smailagić, DijanaDragišić Maksimović, Jelena
Stupar, Sofija
Banjac, Nevena
Ninković, Slavica
Stanišić, Mariana
Остала ауторства
Milutinović, MilicaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2022 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Apple species are exclusively rich source of dihydrochalcones phloretin and phloridzin, phenolic compounds with elusive role in planta but suggested auto-allelochemical features related to
‘apple replant disease’ (ARD). Our recent study emphasized phytotoxic action of phloretin towards
other plant species such as model plant Arabidopsis as a consequence of disturbed auxin homeostasis in roots and altered chloroplast structure and function. Since elevated production and
accumulation of reactive oxygen species (ROS) in plant cells is the one of the most common effect
of allelochemicals, this study aimed to analyze the antioxidant status of Arabidopsis seedlings
treated with phloretin. Total phenolic content (TPC) and total antioxidant capacity (TAC), as well
as antioxidant enzymes activities were determined spectrophotometrically in treated and control
seedlings at 5th and 10th day after germination (DAG). TAC increased c.a. 280% and 200% relative to
control at 5th and 10th DAG respectively, presumably due to highly elevated TPC in treated seedlings (c.a. 150%). At the same time, the activity of antioxidant enzymes significantly decreased. The
activity of catalase (CAT) was reduced by c.a. 40% and guaiacol peroxidase (POD) activities by 93%
and 47% at 5th and 10th DAG, respectively. The activity of superoxide dismutase (SOD) was reduced
by 55% and 18% at 5th and 10th DAG, respectively. The burst of antioxidant capacity of phloretin
treated Arabidopsis seedlings resulted in significantly decreased antioxidant enzymes activities
presumably due to strong antioxidant properties of absorbed phloretin. However, phloretin inhibition of antioxidant enzymatic activity due to its direct interaction with enzymes is object of
further investigation.
Кључне речи:
antioxidant enzymes; Malus × domestica Borkh.; phloretin; phytotoxicity; total phenolic contentФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Milutinović M, editor. Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 73.