Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells.
2022
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Authors:
Trifunović, SaraSmiljanić, Katarina
Sickmann, Albert
Solari, Fiorella A.
Kolarević, Stoimir
Divac Rankov, Aleksandra
Ljujić, Mila
Document Type:
Article (Published version)
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Show full item recordAbstract:
BACKGROUND Although still considered a safer alternative to classical cigarettes, growing body of work points to harmful effects of electronic cigarettes (e-cigarettes) affecting a range of cellular processes. The biological effect of e-cigarettes needs to be investigated in more detail considering their widespread use. METHODS In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration of e-cigarette liquids, with and without nicotine. Mutagenicity was evaluated by HPRT assay, genotoxicity by comet assay and the effect on cellular communication by metabolic cooperation assay. Additionally, comprehensive proteome analysis was performed via high resolution, parallel accumulation serial fragmentation-PASEF mass spectrometry. RESULTS E-cigarette liquid concentration used in this study showed no mutagenic or genotoxic effect, however it negatively impacted metabolic cooperation between V79 cells. Both e-cigarette liquids induced significant depletion in total number of proteins and impairment of mitochondrial function in treated cells. The focal adhesion proteins were upregulated, which is in accordance with the results of metabolic cooperation assay. Increased presence of posttranslational modifications (PTMs), including carbonylation and direct oxidative modifications, was observed. Data are available via ProteomeXchange with identifier PXD032071. CONCLUSIONS Our study revealed impairment of metabolic cooperation as well as significant proteome and PTMs alterations in V79 cells treated with e-cigarette liquid warranting future studies on e-cigarettes health impact.
Keywords:
Electronic cigarettes; Lung fibroblast; Metabolic cooperation; ProteomicsSource:
Respiratory Research, 2022, 23, 1, 191-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200168 (University of Belgrade, Faculty of Chemistry) (RS-MESTD-inst-2020-200168)
- FoodEnTwin-Twinning of research activities for the frontier research in the fields of food, nutrition and environmental omics (EU-H2020-810752)
- Mediterranean Institute for Life Sciences (MedILS NaosILS Strategic Alliance Agreement), Split, Croatia
- Bundesministerium fur Bildung und Forschung (BMBF)
- Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen (MKW)
- Regierender Burgermeister von Berlin
DOI: 10.1186/s12931-022-02102-w
ISSN: 1465-993X
PubMed: 35840976
WoS: 000826047400001
Scopus: 2-s2.0-85134238387
URI
https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-022-02102-whttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9285873
http://radar.ibiss.bg.ac.rs/handle/123456789/5082