Phenethyl ester of rosmarinic acid ameliorates experimental autoimmune encephalomyelitis
2022
Autori:
Stegnjaić, GoranLazarević, Milica
Diamantis, Dimitrois A
Nikolovski, Neda
Jevtić, Bojan
Stanisavljević, Suzana
Dimitrijević, Mirjana
Momčilović, Miljana
Tzakos, Andreas G
Miljković, Đorđe
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2022 European Federation of Immunological Societies. Published by Elsevier B.V.
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Rosmarinic acid is a polyphenolic compound, abundantly present in herbs of the Lamiaceae family. The aim of
the study was to evaluate the immunomodulatory properties of a recently developed phenethyl ester derivative
of rosmarinic acid (PERA), with enhanced ability of diffusion through biological membranes, in an animal model
of the central nervous system (CNS) autoimmunity. To this end, experimental autoimmune encephalomyelitis
(EAE), an animal model of multiple sclerosis was used. Daily subcutaneous administration of PERA (30 mg/kg)
from day 7 to day 22 after immunization successfully ameliorated EAE induced in Dark Agouti rats, shortening
the disease duration and reducing maximal, cumulative and mean clinical score. PERA efficiently reduced
production of major encephalitogenic cytokines, interferon (IFN)-γ and interleukin (IL)-17, in immune cells from
the CNS or the lymph nodes draining the site of immunization of EAE rats, as well as in CD4+ T cells purified
from the lymph nodes. Also, PERA inhibited NO production in the CNS and the lymph nodes, as well as in
macrophages and microglial cells. Finally, microglial ability to produce pro-inflammatory cytokines IL-6, and
tumor necrosis factor (TNF) were also reduced by PERA. Our results clearly imply that PERA possesses antiencephalitogenic properties. Thus, further studies on the relevance of the observed effects for the therapy of
multiple sclerosis are warranted.
Ključne reči:
Experimental autoimmune encephalomyelitis; Rosmarinic acid; T cells; Cytokines; Multiple sclerosisIzvor:
Immunology Letters, 2022, 251-252, 9-19Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Hellenic Foundation for Research and Innovation “First Call for H.F.R.I. Research Projects to support Faculty members and Researchers and the procurement of high-cost research equipment grant” (No. 991)
- “Infrastructure of Microbiome Applications in FoodSystems-FOODBIOMES” (MIS 5,047,291)
DOI: 10.1016/j.imlet.2022.09.006
ISSN: 0165-2478
PubMed: 36183900