Anticancer Potential of Xanthohumol and Isoxanthohumol Loaded into SBA-15 Mesoporous Silica Particles against B16F10 Melanoma Cells.
2022
Authors:
Krajnović, TamaraPantelić, Nebojša Đ.
Wolf, Katharina
Eichhorn, Thomas
Maksimović-Ivanić, Danijela
Mijatović, Sanja
Wessjohann, Ludger A.
Kaluđerović, Goran N.
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Xanthohumol (XN) and isoxanthohumol (IXN), prenylated flavonoids from Humulus lupulus, have been shown to possess antitumor/cancerprotective, antioxidant, antiinflammatory, and antiangiogenic properties. In this study, mesoporous silica (SBA-15) was loaded with different amounts of xanthohumol and isoxanthohumol and characterized by standard analytical methods. The anticancer potential of XN and IXN loaded into SBA-15 has been evaluated against malignant mouse melanoma B16F10 cells. When these cells were treated with SBA-15 containing xanthohumol, an increase of the activity correlated with a higher immobilization rate of XN was observed. Considering the amount of XN loaded into SBA-15 (calculated from TGA), an improved antitumor potential of XN was observed (IC50 = 10.8 ± 0.4 and 11.8 ± 0.5 µM for SBA-15|XN2 and SBA-15|XN3, respectively; vs. IC50 = 18.5 ± 1.5 µM for free XN). The main mechanism against tumor cells of immobilized XN includes inhibition of proliferation and autophagic cell death. The MC50 values for SBA-15 loaded with isoxanthohumol were over 300 µg/mL in all cases investigated.
Keywords:
SBA-15; Cytotoxicity; Isoxanthohumol; Melanoma; XanthohumolSource:
Materials (Basel, Switzerland), 2022, 15, 14, 5028-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200116 (University of Belgrade, Faculty of Agriculture) (RS-MESTD-inst-2020-200116)
- Deutscher Akademischer Austausch Dienst (DAAD) 57448219
DOI: 10.3390/ma15145028
ISSN: 1996-1944
PubMed: 35888494
WoS: 000831513200001
Scopus: 2-s2.0-85137269977
URI
https://www.mdpi.com/1996-1944/15/14/5028http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9320346
http://radar.ibiss.bg.ac.rs/handle/123456789/5089