Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization
2023
Authors:
Popović Minić, DušankaMilinčić, Danijel
Kolašinac, Stefan
Rac, Vladislav
Petrović, Jovana
Soković, Marina
Banjac, Nebojša
Lađarević, Jelena
Vidović, Bojana
Kostić, Aleksandar
Pavlović, Vladimir
Pešić, Mirjana
Document Type:
Article (Published version)
,
© 2022 Elsevier Ltd.
Metadata
Show full item recordAbstract:
This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.
Keywords:
Goat milk; Proteins; Agaricus blazei; DLS measurements; FTIR; Electrophoresis; SEMSource:
Food Chemistry, 2023, 402, 134299-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200116 (University of Belgrade, Faculty of Agriculture) (RS-MESTD-inst-2020-200116)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200116 (University of Belgrade, Faculty of Agriculture) (RS-MESTD-inst-2020-200116)
- FUNPRO - Functional products based on goat's milk proteins and bioactive compounds extracted from grape pomace and edible mushrooms (RS-ScienceFundRS-Ideje-7744714)
DOI: 10.1016/j.foodchem.2022.134299
ISSN: 0308-8146
PubMed: 36152557