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Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage.
dc.creator | Radovanović, Jelena | |
dc.creator | Antonijević, Biljana | |
dc.creator | Ćurčić, Marijana | |
dc.creator | Baralić, Katarina | |
dc.creator | Kolarević, Stoimir | |
dc.creator | Bulat, Zorica | |
dc.creator | Đukić-Ćosić, Danijela | |
dc.creator | Buha Đorđević, Aleksandra | |
dc.creator | Vuković-Gačić, Branka | |
dc.creator | Javorac, Dragana | |
dc.creator | Antonijević Miljaković, Evica | |
dc.creator | Carević, Momir | |
dc.creator | Mandinić, Zoran | |
dc.date.accessioned | 2022-11-18T08:39:49Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2022 | |
dc.identifier.issn | 0269-7491 | |
dc.identifier.uri | http://radar.ibiss.bg.ac.rs/handle/123456789/5166 | |
dc.description.abstract | Excessive fluoride (F-) levels in the environment could induce different pathological changes, including comorbidities in reproductive functions. Hence, the aim of the present in vivo study was to explore F- subacute toxicity mechanisms via Benchmark dose (BMD) methodology on rat's testicles. The experiment was conducted on thirty male Wistar rats for 28 days, divided into six groups (n = 5): 1) Control (tap water); 2) 10 mg/L F-; 3) 25 mg/L F-; 4) 50 mg/L F-; 5) 100 mg/L F-; 6) 150 mg/L F-. Testicles were dissected out and processed for the determination of F- tissue concentrations, redox status parameters, essential elements level, and DNA damage. PROASTweb 70.1 software was used for determination of external and internal dose-response relationship. The results confirmed a significant increase in superoxide anion (O2.-), total oxidative status (TOS), copper (Cu), zinc (Zn), iron (Fe), DNA damage levels, and decrease in superoxide dismutase activity (SOD1) and total thiol (SH) groups. The dose-dependent changes were confirmed for SOD1 activity and DNA damage. The most sensitive parameters were SOD1 activity and DNA damage with the lowest BMDLs 0.1 μg F-/kg b. w. Since human and animal populations are daily and frequently unconsciously exposed to F-, this dose-response study is valuable for further research regarding the F- health risk assessment. | |
dc.publisher | Elsevier Ltd. | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200017/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200017/RS// | |
dc.rights | restrictedAccess | |
dc.source | Environmental Pollution | |
dc.subject | Benchmark methodology | |
dc.subject | Bioelements | |
dc.subject | Comet assay | |
dc.subject | Fluoride | |
dc.subject | Oxidative stress | |
dc.subject | Testicles | |
dc.title | Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage. | |
dc.type | article | en |
dc.rights.license | ARR | |
dc.rights.holder | © 2022 Elsevier Ltd | |
dc.citation.volume | 314 | |
dc.identifier.doi | 10.1016/j.envpol.2022.120321 | |
dc.identifier.pmid | 36191801 | |
dc.identifier.scopus | 2-s2.0-85139290520 | |
dc.identifier.wos | 000882962000007 | |
dc.citation.apa | Radovanović, J., Antonijević, B., Ćurčić, M., Baralić, K., Kolarević, S., Bulat, Z., et al. (2022). Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage. Environmental Pollution, 314, 120321. | |
dc.citation.vancouver | Radovanović J, Antonijević B, Ćurčić M, Baralić K, Kolarević S, Bulat Z, Đukić-Ćosić D, Buha Djordjević A, Vuković-Gačić B, Javorac D, Antonijević Miljaković E, Carević M, Mandinić Z. Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage. Environ Pollut. 2022;314:120321. | |
dc.citation.spage | 120321 | |
dc.type.version | publishedVersion | |
dc.citation.rank | aM21 |