Cellular redox homeostasis mediates contractility of isolated ileum of male Wistar rats
2022
Autori:
Grahovac, TanjaVidonja Uzelac, Teodora
Nikolić-Kokić, Aleksandra
Oreščanin Dušić, Zorana
Blagojević, Duško
Ostala autorstva
De La Rosa, MiguelTip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2022 The Authors. FEBS Open Bio 2022 FEBS
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Redox-mediated mechanisms play an important role in regulating
smooth muscle contractility and depend on subtle redox homeostasis
maintaining. Redox homeostasis in cells is obtained by
the activity of antioxidant enzymes. Among them, glutathione
reductase constantly reduces oxidized glutathione, providing a
favorable cellular redox milieu. The aim of this study was to
examine whether, to what extent and in what way changes in
smooth muscle contractility occurred in conditions when glutathione
reductase activity was changed. The experiments were
performed on isolated ileum of male Wistar rats, electrically stimulated
and treated with cumulative doses of carmustine, a glutathione
reductase inhibitor. The activity of antioxidant enzymes(catalase, glutathione reductase and glutathione peroxidase, as
well as the amount of total SH groups and glutathione) was
determined in treated ileum. The addition of carmustine inhibited
glutathione reductase activity and decreased amplitude and frequency
of electrically stimulated ileum. The results showed that
glutathione reductase activity and maintenance of highly reductive
cellular environment were essential to contractility processes.
U:
- De La Rosa M, editor. The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal. Hoboken : John Wiley & Sons Ltd; 2022. p. 220-1. (FEBS Open Bio; Vol. 12; Suppl. 1).