Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice
2022
Authors:
Ćirić, JelenaTešić, Vesna
Milovanović, Nikola
Jovanović Macura, Irena
Kanazir, Selma
Perović, Milka
Contributors
De La Rosa, MiguelDocument Type:
Conference object (Published version)
,
© 2022 The Authors. FEBS Open Bio 2022 FEBS
Metadata
Show full item recordAbstract:
Neuroprotective effects of food restriction were demonstrated in
several animal models of stroke, traumatic brain injury, and neurodegenerative
diseases. Since Alzheimer’s disease (AD) is characterized
by a long silent prodromal phase, the present study
aimed to determine the effects of every-other-day (EOD) feeding
on cortical responsiveness to PV interneurons and BDNF/TrkB
signaling using 5xFAD mice, a well-characterized mouse model
of AD. Female 5xFAD transgenic (Tg) mice and their non-transgenic
littermates were exposed to ad libitum (AL) or EOD feeding
regimen, beginning at 2 months of age. Neurons expressing
PV were detected in the retrosplenial dysgranular cortex (RSGc),
retrosplenial granular cortex (RSD), parietal cortex (PtA), and
somatosensory cortex (S) of 6-month-old animals by immunohistochemistry.
Analysis of the BDNF/Trk signaling was examined
by western blot. TgAL mice showed a significantly reduced number
of PV-positive cells in the RSGc, PtA, and S, while no
changes were detected in the RSD. Interestingly, four months of
EOD feeding reverted the number of PV-positive cells to control
values in all three regions examined. BDNF was decreased in the
TgAL mice, which was additionally decreased in TgEOD mice,
while no significant difference in pro-BDNF was identified. Analysis
of TrkB and pTrkB revealed a significant increase of TrkB
in the TgEOD group, whereas a significant decrease in pTrkB
was detected only in the TgAL group. The present study indicates
that every-other-day feeding can ameliorate PV neuronal
loss, and have an important role in further understanding the
neural basis of AD-like-associated cognitive impairments in
5xFAD mouse model of AD.
In:
- De La Rosa M, editor. The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal. Hoboken : John Wiley & Sons Ltd; 2022. p. 132. (FEBS Open Bio; Vol. 12; Suppl. 1).