Bioactivity of different Asplenium ceterach L. extracts
2022
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Authors:
Živković, SuzanaMilutinović, Milica
Gašić, Uroš
Ivanov, Marija
Ćirić, Ana
Banjanac, Tijana
Skorić, Marijana
Contributors
Milutinović, MilicaDocument Type:
Conference object (Published version)
,
© 2022 by the Serbian Plant Physiology Society
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Show full item recordAbstract:
Methanol (ME) and dichloromethane (DCM) extracts of rustyback fern (Asplenium ceterach L.) sporophytes were analyzed for antioxidant properties by thin layer chromatography (TLC)-based
bioautography, using DPPH as detection reagent, and comparatively tested for their antimicrobial and antibiofilm activity. Bioautography assay indicated that rustyback fern ME extract had signif-
icant antioxidant potential and moderate α-amylase activity, in comparison to the other extract
used in the study. Antibacterial and antifungal activity of plant extracts against 6 bacteria and 6
fungi strains was assessed by microdilution method and the results are presented as minimal in-
hibitory concentration and minimal bactericidal/fungicidal concentration. Both extracts showed
significant antibacterial and antifungal potential against all tested strains, although in variable
degree. The Gram-negative Escherichia coli and Gram-positive Bacillus cereus were the most sen-
sitive bacteria, whereas the most sensitive fungus was yeast Candida krusei. Furthermore, tested
extracts have shown significant capability to prevent Candida albicans biofilm establishment with
no significant difference between the two extracts examined, and inhibition range of 72%–74%.
The results of the present study highlight A. ceterach extracts as a significant source of bioactive
com-pounds, which support their potential use against diseases caused by multi-drug resistant
microorganisms.
Keywords:
rustyback fern; antimicrobial activity; antioxidative potentialFunding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
In:
- Milutinović M, editor. Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 128.