Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts
2022
Аутори:
Ajduković, MajaVučić, Tijana
Cvijanović, Milena
Filipović, Branko
Šošić-Jurjević, Branka
Остала ауторства
Crnobrnja-Isailović, JelkaVukov, Tanja
Vučić, Tijana
Tomović, Ljiljana
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2022 Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
Метаподаци
Приказ свих података о документуАпстракт:
Aquatic ecosystems receive the majority of global pollution. Therefore, aquatic
organisms are the most exposed and the most vulnerable as penetration of various
endocrine disruptors is easier, resulting in high bioavailability and bioaccumulation of
chemicals. One of the potent endocrine disruptors (anti-thyroid agents) is thiourea,
which chemically blocks the synthesis of thyroid hormones and prevents
metamorphosis in amphibians. The mechanism of action is very similar for all antithyroid agents via inhibition of thyroid peroxidase enzyme (TPO), thereby inhibiting
iodination of tyrosine residues in thyroglobulin, the oxidative coupling of iodinated
tyrosine and therefore the biosynthesis of the thyroid hormones. We investigated the
influence of two non-lethal concentrations of thiourea (0.05% and 0.1%) on
histomorphology of the thyroid gland in Triturus newts at the metamorphic stage,
when TH concentrations should reach maximum level. Exposure to thiourea induced
hypertrophy and hyperplasia of follicular cells as well as a significant reduction
(p<0.05) of interstitial tissue. Intensity of thyroglobulin immunostaining significantly
decreased (p<0.05) upon both thiourea treatments. Additionally, we tested
mammalian thyroglobulin antibodies for immunohistochemical assessment of the
thyroid follicular tissue in newts. Successful cross-reactivity of human primary
antibody in immunochemical detection of thyroglobulin in Triturus newts in this study
confirms potential homology in protein structure throughout the vertebrates.
У:
- Crnobrnja-Isailović J, Vukov T, Vučić T, Tomović Lj, editors. Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade; 2022. p. 41.