Craniofacial development of Triturus newts – a suitable model system for testing the developmental hourglass model
2022
Аутори:
Bugarčić, MarkoIvanović, Ana
Cvijanović, Milena
Ajduković, Maja
Wielstra, Ben
Vučić, Tijana
Остала ауторства
Crnobrnja-Isailović, JelkaVukov, Tanja
Vučić, Tijana
Tomović, Ljiljana
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2022 Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
Метаподаци
Приказ свих података о документуАпстракт:
In developmental biology a plethora of studies support the existence of a
conserved stage during the ontogeny of vertebrates – the pharyngula. The
developmental hourglass model predicts that the most conserved morphological
pattern occurs in the middle of embryonic development. We explored external
morphology of the craniofacial region of Triturus newts using 3D geometric
morphometrics. The craniofacial region was selected because it experiences
pleiotropic developmental constrains due to its major role in feeding, respiration and
the housing of substantial parts of nervous and sensory systems. We aimed to uncover
differences in variance between successive developmental stages, because it is
proposed that the more constrained stages should have the lowest variance. In
addition, we explored differences in the craniofacial shape between successive
developmental stages. Stages included start from the moment after the completion of
neurulation and span the mid-tailbud phase. The least amount of variance in shape was
recorded at stage 24 (according to D’Amen and colleagues). In general, this stage is
characterized by changes in overall head shape, the distinction of optical vesicles,
intensive somitogenesis and formation of the tailbud which overgrows the blastopore,
indicating that these processes could be highly constrained. Only significant
craniofacial shape change occurred between this and successive stage. The most
pronounced differences were due to growing of the head region and gill bud balancers.
Optic vesicles became concave due to inductive processes preceding eye formation.
These preliminary results indicate that stage 24 could be the most conserved one
during early ontogeny as it is preceded and followed by stages exhibiting more
variance in shape. Our results indicate that the craniofacial development represents a
suitable model system for testing the hourglass model of development. Larger sample
size, exploration of the entire embryonic development and ontogeny of additional newt
species is needed to confirm our preliminary findings.
У:
- Crnobrnja-Isailović J, Vukov T, Vučić T, Tomović Lj, editors. Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade; 2022. p. 42.