Glucocorticoid-mediated effects of MIF deficiency and fructose-enriched diet on lipid metabolism in the mouse intra-abdominal adipose tissue
2017
Preuzimanje 🢃
Autori:
Gligorovska, LjupkaĐorđević, Ana
Bursać, Biljana
Teofilović, Ana
Veličković, Nataša
Vojnović-Milutinović, Danijela
Kovačević, Sanja
Matić, Gordana
Ostala autorstva
Brajušković, GoranĐorđević, Ana
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2017 University of Belgrade, Faculty of Biology
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Introduction: The macrophage migration inhibitory factor (MIF) is a pro-inflammatory
cytokine involved in metabolic inflammation and regulation of glucocorticoid
action. Low-grade inflammation in adipose tissue is associated with obesity and
dyslipidemia and may be caused by fructose-enriched diet. We hypothesized that
the effects of MIF deficiency on lipid metabolism in adipose tissue, after high fructose
consumption, could be mediated by glucocorticoids (GCs) as potent regulators of
energy metabolism.
Methods: We analyzed the effects of 9-week 20% fructose-enriched diet on energy
intake, body mass, intra-abdominal adipose tissue mass and histology in MIF wild
type (WT) and MIF deficient (MIF−/−) C57Bl/6J mice. Expression of key transcriptional
regulators involved in adipogenesis and lipogenesis, peroxisome-proliferator-
activated receptor γ (PPARγ) and sterol regulatory element-binding protein-1
(SREBP-1), was also assessed. Glucocorticoid signaling was characterized by
prereceptor metabolism, glucocorticoid receptor (GR) protein level and
phosphorylation, and expression of GC-target genes involved in lipogenesis.
Results: Both WT and MIF−/− mice on fructose diet had increased energy intake, but
the elevation of adipose tissue mass and enlargement of adipocytes were observed
only in fructose-fed MIF−/− mice. Elevated GR protein level and its activating Ser220
phosphorylation, enhanced glucocorticoid prereceptor metabolism, an increase in
PPARγ and SREBP-1 levels and induced expression of all examined lipogenic genes
were also observed in MIF−/− mice on fructose diet.
Conclusion: The results show that only under fructose caloric overload MIF deficiency
results in lipogenesis and adipocyte hyperthrophy and that this effect might be
mediated by enhanced glucocorticoid signalling in intra-abdominal adipose tissue
Finansiranje / projekti:
- Uloga steroidnih hormona u neuroendokrinoj adaptaciji na stres i patofiziologiji metaboličkog sindroma - molekularni mehanizmi i kliničke implikacije (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
U:
- Brajušković G, Đorđević A, editors. CoMBoS. Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia. Belgrade: University of Belgrade, Faculty of Biology; 2017. p. 38.