(Micro)Plastic pollution in freshwater ecosystems- a model organism
2022
Аутори:
Stanković, JelenaPaunović, Momir
Milošević, Đurađ
Tomović, Jelena
Ilić, Marija
Đuknić, Jelena
Čanak Atlagić, Jelena
Raković, Maja
Остала ауторства
Joksimović, AleksandarТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2022 by the University of Montenegro, Institute of Marine Biology
Метаподаци
Приказ свих података о документуАпстракт:
Plastic pollution is recognized as ubiquitous and different approaches have been used
to detect and evaluate effects on aquatic biota. Here, we present Asian clam Corbicula
fluminea (Müller, 1774) as a suitable model organism for detecting microplastic
pollution in freshwater ecosystems. The study was conducted on the Danube River and
its tributaries. Following the multi-habitat procedure, specimens were sampled using a
hand net (ap. 25 cm × 25 cm, mesh size 500 μm) sampling. Out of a total of 51 sampling
sites, C. fluminea was collected at 23 sites, whereas 15 sites were on the Danube and
8 sites on its tributaries. From each site 10 specimens were randomly selected. The
shell length, shell width, total weight and body weight were measured. The samples
were digested by alkaline method, using a KOH 10% solution and incubation at 65 ⁰C
for 12 h. Collected particles were photographed and assigned to one of 2 major
microplastic categories: fibers and fragments. Categories were divided in
subcategories based on the coloration of the particles. Particles were counted manually,
photographed and measured by the use of Nikon SMZ 745T Stereomicroscope. All
collected particles were measured in the program ImageJ. Microplastic particles were
detected in all samples of C. fluminea, on average 5.59 ± 3.71 fibrils and 4.37 ± 2.46
fragments per organism; or 40.77 ± 73.75 fibrils and 25.84 ± 33.17 fragments per g
body weight, 1998 in total. Medium-sized microplastic particles were dominant, with
an average length of 0.43 mm ± 0.26 in the Danube and 0.49 mm ± 0.26 in the
tributaries. Among fibers and fragments, blue fibers (81%) and transparent fragments
(42.8%) were dominant. In order to confirm chemical composition of isolated
microliter, 46 particles of the hard plastic from 14 sampling sites were analyzed with
micro- FTIR spectroscopy analysis. Analyzed particles were detected as polyethyleneterephthalate
and cellophane, most commonly.
Кључне речи:
microplastic pollution; model organism; Asian clam; Danube; microFTIR spectroscopyУ:
- Joksimović A, editor. Book of Abstracts: International Conference Adriatic Biodiversity Protection AdriBioPro2022; 2022 Jun 13-17; Kotor, Montenegro. Kotor, Montenegro : University of Montenegro, Institute of Marine Biology; 2022. p. 91.