Long term effects in dwarf bearded iris (Iris reichenbachii Heuff.) calli metabolism induced by plasma treatment
2022
Authors:
Živković, SuzanaJevremović, Slađana
Gašić, Uroš
Milutinović, Milica
Petrović, Zoran Lj.
Škoro, Nikola
Puač, Nevena
Contributors
Hensel, KarolCimerman, Richard
Lavrikova, Aleksandra
Janda, Mário
Machala, Zdenko
Document Type:
Conference object (Published version)
,
© 2022 by FMFI UK
Metadata
Show full item recordAbstract:
The Plasma Agriculture is a new field of plasma applications where non-thermal (cold)
plasmas (NTPs) operating at atmospheric pressure are used as a tool in biotechnology for genetic
manipulation of plants, for micropropagation, for studies of plant metabolism and cellular
development or a commercial production of natural products that cannot be chemically synthesized.
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are responsible
for triggering various mechanisms and effects in plant cells, such as the induction of somatic
embryogenesis, higher and faster seed germination, better water uptake or have an anti-bacterial and
anti-viral effects, etc. [1-3]. In the current study plant undifferentiated compact tissue (calli) of
Balkan endemic dwarf bearded iris (Iris reichenbachii Heuff.) was treated using a RF plasma
needle device operating with He as a working gas. The flow of He was kept constant at 1 slm and
the power deposited to the plasma was below 2 W. The plasma needle was positioned 3 mm above
the callus surface enabling direct contact between the active plasma volume and the surface of the
sample. We induced significant morphological alterations in structure of non-embryonic calli that
could be attributed to the enhanced cell division of the plant cells at the surface of the calli that was
in contact with plasma. The differentiation of the calli cells was stimulated by reactive species
created in gas phase of NTP. The morphological changes were then followed by the significant long
term alteration in specialized metabolite content in derived calli types. Our results implicate that
direct plasma treatment could serve as a significant elicitor of the production of specific metabolites
in dwarf bearded iris calli.
Keywords:
non-thermal plasma; RF plasma needle; calli; iris; specialized metabolitesFunding / projects:
- COST Action CA19110 - PlAgri, supported by COST (European Cooperation in Science and Technology-www.cost.eu
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200024 (University of Belgrade, Institute of Physics, Belgrade-Zemun) (RS-MESTD-inst-2020-200024)
In:
- Hensel K, Cimerman R, Lavrikova A, Janda M, Machala Z, editors. Book of Abstracts: 9th Central European Symposium on Plasma Chemistry (CESPC-9) joint with COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri); 2022 Sep 4-9; Vysoké Tatry, Slovakia. Bratislava: FMFI UK; 2022. p. 137.