Tissue-specific distribution of antioxidants during ripening of Solanum dulcamara L. fruits: the redox state alterations
2022
Authors:
Milutinović, MilicaNakarada, Đura
Božunović, Jelena
Gašić, Uroš
Živković, Suzana
Savić, Jelena
Skorić, Marijana
Devrnja, Nina
Banjanac, Tijana
Todorović, Miloš
Mojović, Miloš
Mišić, Danijela
Contributors
Milutinović, MilicaDocument Type:
Conference object (Published version)
,
© 2022 by the Serbian Plant Physiology Society
Metadata
Show full item recordAbstract:
Identifying plant species tolerant to hypoxia and determining mechanisms this tolerance is standing upon may aid in improving nutritional quality of fruits of waterlogging-intolerant crops. Berries of Solanum dulcamara L. (bittersweet), a Eurasian species belonging to the Solanaceae family that grows in both dry habitats and wetlands, represent a suitable hypoxia-tolerant model species to study redox processes in relation to fruit development. Using biochemical and physicochemical analyses, we examined differences in enzymatic and non-enzymatic components of the antioxidant system of S. dulcamara during the transition from mature green (MG) to ripe red (RR) developmental stage. By combining untargeted (UHPLC/Orbitrap MSn) and targeted (UHPLC-DAD MS2) metabolomics approaches, we revealed a significant change in polyphenolic profile of S. dulcamara fruits during fruit ripening. In order to improve knowledge on the link between metabolism and cell redox status, Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) of reactive oxygen species (ROS) in intact fruits, fruit sections and extracts, were performed in parallel with the analysis of tissue-specific distribution of antioxidants and activity of antioxidant enzymes, which demonstrated alterations in the redox state and the increase of total antioxidant capacity of bittersweet berries during ripening, especially of outer layers (pericarp) of the fruits. This knowledge could provide important information to direct research on fruit quality improvement of flood-intolerant crops that are a close relatives of bittersweet, such as tomato and eggplant.
Keywords:
waterlogging; fruit; bittersweet; antioxidant system; metabolomicsFunding / projects:
- "Oxygen sensing a novel mean for biology and technology of fruit quality” (CA18210) which is implemented under the COST Action “Roxy-COST
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200146 (University of Belgrade, Faculty of Physical Chemistry) (RS-MESTD-inst-2020-200146)
In:
- Milutinović M, editor. Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 126.