Antitumor properites of alchemilla vulgaris agg.
2022
Аутори:
Jelača, SanjaDrača, Dijana
Dajić-Stevanović, Zora
Jovanović, Ivan
Tanić, Nikola
Mijatović, Sanja
Arsenijević, Nebojša
Maksimović-Ivanić, Danijela
Остала ауторства
Arsenijević, NebojšaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2022 by the Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine
Метаподаци
Приказ свих података о документуАпстракт:
Alchemilla vulgaris is well known in traditional medicine especially for the treatment
of gynecological problems in women. Several ethnomedicinal studies for the territory
of Balkan reports its well known biological properties against many conditions such
as infertility, dysmenorrhea, cysts, menopausal complaints and endometriosis.
Concidering ethnomedicinal data on female illnesses, the aim of our study was to
determine whether ethanol extract of Alchemilla vulgaris agg. exert antitumor effect
against mouse breast cancer cells in vitro. Treatment with Alchemilla vulgaris agg. extract
decreased viability of mouse breast cancer cells (4T1) in dose-dependent manner after
72 h. The viability decrease was followed by loss of dividing potential after the treatment.
In parallel with this, certain percentage of 4T1 cells was subjected to programmed cell
death ̶ apoptosis. Detected apoptosis was followed with caspase activation while typical
apoptotic morphology of treated cells was observed by fluorescent microscopy. Apart
from inhibited cell division and induced apoptosis, decreased cell viability was due to
triggered autophagy cell death. In parallel, diminished metastatic potential of these
cells was confirmed by abrogated adhesion, invasion, migration and decreased colony
forming potential after the treatment. All mentioned effects can be connected with
enhanced production of ROS and intracellular NO after the treatment with Alchemilla
vulgaris agg. Taken together, the effect of Alchemilla vulgaris agg. against breast cancer
cells makes this plant worthwhile for further evaluation in the field of oncology.
Кључне речи:
Alchemilla vulgaris agg.; breast cancer; metastasisФинансирање / пројекти:
- Joint funding of Development and research projects of the Republic of Serbia and the People’s Republic of China
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Arsenijević N, editor. Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina. Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine; 2022. p. 129.