New aspects of synthetic tubulysin derivative, tubugi 1, action in murine melanoma model
2022
Аутори:
Drača, DijanaMijatović, Sanja
Krajnović, Tamara
Bogdanović Pristov, Jelena
Đukić, Tatjana
Kaluđerović, Goran N.
Wessjohann, Ludger A.
Maksimović-Ivanić, Danijela
Остала ауторства
Arsenijević, NebojšaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2022 by the Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine
Метаподаци
Приказ свих података о документуАпстракт:
Tubulisins are natural peptide compounds isolated from mycobacterial genera.
They belong to the group of antimitotic agents, since they achieve their antitumor
effect disrupting the function of mitotic spindle. The object of this study was to investigate
anticancer potential of synthetic analogue of tubulysins, tubugi 1, on mouse
melanoma model, in vitro and in vivo. Tubugi 1 decreased dose-dependently viability
of B16 cells. The experimental compound induced atypical apoptosis without the externalization
of phosphatidylserines (PS). Although apoptosis was accompanied with
strong intracellular production of reactive oxygen and nitrogen species, decrease in
malonyldyaldehyde content showed that membrane lipids were not subjected to oxidation,
what is a prerequisite for the externalization of PS. Although PS plays a key
role in the removal of apoptotic cells, this did not affect the phagocytic activity of
macrophages in vitro, implying PS-independent apoptotic cells removal. The effect of
the experimental agent was confirmed in vivo. Мacrophages isolated from peritoneal
exudate of treated animals showed cytotoxic activity, what was in line with demonstrated
expression of M1 phenotype markers, as well as production of nitric oxide.
Additonally, the phagocytic activity of these cells was preserved. Having in mind lack
of data in the literature concerning the effects of this group of agents on components
of the innate immune system, tubugi 1 remains worthy of further research in the field
of experimental oncology.
Кључне речи:
apoptosis; cancer; phagocytosis; phosphatidylserineФинансирање / пројекти:
- Ministry of Education, Science and Technological Development of the Republic of Serbia
- Leibniz Institute of Plant Biochemistry, Halle
- German Academic Exchange Service (DAAD)
У:
- Arsenijević N, editor. Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina. Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine; 2022. p. 41-2.