Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats
2022
Autori:
Tucović, DinaKulaš, Jelena
Mirkov, Ivana
Popović, Dušanka
Zolotarevski, Lidija
Despotović, Marta
Kataranovski, Milena
Popov Aleksandrov, Aleksandra
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
©2022 by China CDC
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Objective The effect of oral cadmium (Cd) intake to influence contact skin allergies was examined,
since it is known that Cd is a heavy metal that affects many tissues, including the skin, in which it
disturbs homeostasis, thus resulting in inflammation and injury.
Methods Male rats were evoked with experimental contact hypersensitivity reaction (CHS) to hapten
dinitrochlorobenzene (DNCB), after prolonged (30 day) oral exposure to an environmentally relevant Cd
dose (5 ppm). The ear cell population was analyzed with flow cytometry. Cytokine production by ear
skin cells and the activity of skin-draining lymph node (DLN) cells were measured using enzyme-linked
immunosorbent assay (ELISA).
Results Orally acquired Cd (5 ppm) increased CHS intensity only in Dark Agouti (DA) rats by affecting
inflammatory responses in both the sensitization (an increase of IFN-γ and IL-17 cytokine production)
and challenge (an increase of CD8+ and CD4+ cell number and TNF, IFN-γ and IL-17 cytokine production)
phases. An increased CHS reaction was seen in Albino Oxford (AO) rats only at a high Cd dose (50 ppm),
during the challenge phase (an increase of CD8+ and CD4+ cell number and TNF, IFN-γ and IL-17 cytokine
production).
Conclusion These novel data indicate that oral Cd intensifies the skin response to sensitizing chemicals
such as DNCB.
Ključne reči:
Cadmium (Cd); Contact hypersensitivity reaction (CHS); Dinitrochlorobenzene (DNCB)Izvor:
Biomedical and Environmental Sciences, 2022, 35, 11, 1038-1050Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
DOI: 10.3967/bes2022.132
ISSN: 0895-3988
PubMed: 36443256