Calorie restriction changes the anxiety-like behaviour of ageing male Wistar rats in an onset- and duration-dependent manner
2022
Аутори:
Prvulović, MilicaTodorović, Smilja
Milanović, Desanka
Jović, Milena
Vujović, Predrag
Kanazir, Selma
Mladenović, Aleksandra
Тип документа:
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Although initially recognized as a universally beneficial approach for the prevention of age-related impairments, the outcome of calorie restriction (CR) is now known to depend on several factors, most notably the age of the subject at the CR commencement, and CR duration. We aimed to examine if and how CR affects anxiety-like behaviour when it is introduced at middle age and late middle age. In addition, as the dopaminergic system is one of the main neurotransmitter systems involved in controlling anxiety, we examined the expression of dopamine receptors (D1R, D2R) in the cortex, striatum, and mesencephalon of male Wistar rats of varying ages. The study was performed on rats fed ad libitum (AL) or exposed to calorie restriction (60% of AL intake). Open field and light-dark tests were used to study anxiety-like behaviour, while PCR and Western blot were used to examine the expression of dopamine receptors. Calorie restriction implemented at middle-age led to variable outcomes on anxiety-like behaviour, while CR implemented at late middle age increased anxiety and decreased the availability of D2R levels in the cortex and mesencephalon. Taken together, these results advise caution when implementing calorie restriction late in life. © 2022
Напомена:
This is the peer reviewed version of the following article: Prvulovic M, Todorovic S, Milanovic D, Jovic M, Vujovic P, Kanazir S, Mladenovic A. Calorie restriction changes the anxiety-like behaviour of ageing male Wistar rats in an onset- and duration-dependent manner. Mech Ageing Dev. 2022;204:111666. http://dx.doi.org/10.1016/j.mad.2022.111666
Кључне речи:
Ageing; Anxiety; Behaviour; Calorie restriction; Dopamine receptorsИзвор:
Mechanisms of Ageing and Development, 2022, 204, 111666-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
Повезане информације:
- Друга верзија
https://radar.ibiss.bg.ac.rs/handle/123456789/4946
DOI: 10.1016/j.mad.2022.111666
ISSN: 0047-6374
PubMed: 35331743