Improvement of water defcit stress tolerance of Impatiens walleriana shoots grown in vitro by methyl jasmonate
2022
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Чланак у часопису (Објављена верзија)
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© The Author(s), under exclusive licence to Springer Nature B.V. 2022
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This study was performed to determine the response of Impatiens walleriana, an important horticultural plant, to exogenous
application of methyl jasmonate (MeJA) during in vitro PEG8000−induced water defcit. Shoots were pretreated with graded
MeJA concentrations (0, 5, 50, and 100 µM) in Murashige and Skoog (MS) medium for 7 days. After pretreatment, shoots
were exposed to diferent treatments for 20 days: MS with polyethylene glycol (PEG8000 −0 or 3%), MS with PEG8000
(3%)+MeJA (5, 50 or 100 µM), and MS with MeJA (5, 50 or 100 µM) only. MeJA pretreatment had positive efects on I.
walleriana growth (fresh weight, plant height, number of leaves per plant and proliferation rate) in non-stressed shoots,
especially after application of the lowest MeJA concentration (5 µM). In the water defcit conditions caused by PEG8000, pretreatment with 5 µM MeJA increased all analyzed growth parameters, and caused decreased photosynthetic pigment, proline
and total amino acid content, total polyphenol and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity, hydrogen peroxide and
malondialdehyde content. Additionally, changes in the activity of antioxidant enzymes (superoxide dismutase, peroxidase
and catalase) were induced. Pretreatment with higher MeJA concentrations (50 and 100 µM) mainly suppressed the I. walleriana shoots growth under water defcit, combination treatments and treatment only with MeJA. These results indicate the
potential of MeJA’s in enhancing I. walleriana ability to withstand the water stress in vitro. I. walleriana improved water
stress resistance by promoting defense-related metabolism, which was mainly induced by pretreatment with the lowest
applied MeJA concentration (5 µM).
Кључне речи:
Impatiens walleriana; Methyl jasmonate; Oxidative stress; Polyethylene glycol; Water defcitИзвор:
Plant Cell, Tissue and Organ Culture (PCTOC), 2022
DOI: 10.1007/s11240-022-02432-z
ISSN: 0167-6857