Short term fish oil supplementation changes glial cells function in 5XFAD mice
2022
Autori:
Jović, MilenaMladenović, Aleksandra
Milanović, Desanka
Todorović, Smilja
Prvulović, Milica
Vukojević, Anđela
Simeunović, Valentina
Sokanović, Srđan
Lončarević, Nataša
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2022 by the Federation of European Biochemical Societies
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
The presence of large amounts of amyloid beta (Aβ) oligomers, amyloid plaques formation, and inflammation in the brain are one of the neuropathological characteristics
of Alzheimer's disease (AD). The use of supplements with omega3 fatty acids has been associated with reduced risk and lessened AD pathology. The purpose of this
study was to elucidate whether such a treatment could affects glial cells and macrophages behavior in the early phase of the disease.
We examined influence of fish oil (FO) treatment in 5xFAD mice, an animal model which rapidly recapitulates major hallmarks of AD amyloid pathology. Threemonth
old female 5xFAD mice received FO (100μl/animal/day) via oral gavage during 3 weeks period. Histological analysis was used to detect changes in pathological
features of AD in parietal cortex of 5xFAD mice. Aβ peptide, macrophages, microglial cells and astrocytes were detected by antiAβ42, antiIba1, antiTMEM119
and antiGFAPantibody, respectively. Immunostaining was observed by confocal microscopy. Quantification was done by ImageJ. FO supplementation alters the
behaviour of macrophages prompting them to establish a physical barrier around amyloid plaques and leads to changes in number of over all astrocytes and microglial
cells.
These results confirmed and extended previous findings suggesting that FO supplementation suppresses brain aging and has a typical pleiotropic effect, suggesting that
FO in combination with other drugs could be good approach for longterm treatment in AD suppression.
U:
- Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece. Federation of European Biochemical Societies; 2022.