Protective role of fermented food in LPS-induced inflammation in C57BL/6 mice
2022
Autori:
Vukojević, AnđelaPrvulović, Milica
Todorović, Smilja
Mladenović, Aleksandra
Jović, Milena
Jovanović Macura, Irena
Perović, Milka
Milanović, Desanka
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
,
© 2022 by the Federation of European Biochemical Societies
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Nontransmittable chronic diseases are largely driven by chronic inflammation, which can be connected to poor diet and toxic products of commensal gut microbiome.
Diet intervention can influence gut microbiota function and composition. Fermented foods are specifically known to have antiinflammatory and immunomodulatory
properties, attributed to their high antioxidant content and lactic acidproducing bacteria (LAB)
In this study we examined the effects of sauerkraut brine (SB) on physiological and behavioral responses to systemic inflammation induced by lipopolysaccharides
(LPS) in a mouse model. C57BL/6 mice 90 postnatal days old were used in this study. They were divided into 2 groups and treated with either 150 ml of sauerkraut
brine and pasteurized sauerkraut brine (PSB) for 5 weeks (via oral gavage). Control animals (CON) were receiving an equivalent amount of water. During the final
week of treatment, animals received 5 injections of LPS (0.5 mg/kg, i.p.). Behavior of animals was assessed before and after LPS administration, using the open field
test, lightdark box, Ymaze, tailsuspension and rotarod test. Analysis of proinflammatory brain cytokines has been performed subsequently via Western blot and
PCR. Food consumption and body weight were measured throughout the experiment.
SB and PSB treatments did not influence body weights and behavior compared to CON mice. LPS treatment led to the weight loss and decreased food intake in all
experimental groups. The fastest recovery and a reduced inflammatory response was detected in the SB group. Behavioral analysis revealed differences between three
groups in responses to the LPS challenge.
Ključne reči:
Fermented foods; LypopolysaccharidesU:
- Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece. Federation of European Biochemical Societies; 2022.