The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro
2022
Аутори:
Đurić, MarijaStamenković, Nikola
Trifunović-Momčilov, Milana
Milošević, Snežana
Marković, Marija
Giba, Zlatko
Subotić, Angelina
Остала ауторства
Milutinović, MilicaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2022 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Common centaury (Centaurium erythraea Rafn) is a medicinal plant used in traditional medicine
since ancient times. Centaury inhabits dry pastures, roadsides and fields from lowlands to
mountain vegetation. Considering that centaury can also be found on moderately saline habitats
in nature, it was interesting to investigate the effect of exogenously applied sodium nitroprusside
(SNP), as a donor of nitrogen monoxide (NO), on antioxidative enzymes activity of centaury shoots
grown under stress caused by sodium chloride (NaCl) in vitro. Centaury shoots were grown on
½MS nutrient media containing graded concentrations of SNP (0, 50, 100 and 250 μM). After three
weeks of pretreatment, shoots were further transferred to ½MS nutrient media supplemented
with NaCl (0 or 150 mM) and/or SNP (0, 50, 100 or 250 μM) during one week treatment. After four
weeks, the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT)
and peroxidase (POX) was determined spectrophotometrically. Thus, through 14 different treatments,
the effect of NaCl and SNP on biochemical response of centaury shoots was investigated.
The results obtained in this work showed that NaCl decreased the activity of SOD, CAT and POX.
On the other hand, exogenous application of SNP increased SOD, CAT and POX activity in centaury
shoots after four weeks. It can be concluded that the effect of SNP on increased tolerance of
centaury shoots to salinity is reflected through the changed activity of enzymatic components of
antioxidant defence.
Кључне речи:
centaury; reactive oxygen species; nitric oxide; salinityФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Milutinović M, editor. Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 60.