Light conditions affect the growth, chemical composition, antioxidant and antimicrobial activities of the white-rot fungus Lentinus crinitus mycelial biomass
2022
Authors:
Halabura, Marisangela Isabel WietzikoskiAvelino, Katielle Vieira
Araújo, Nelma Lopes
Kassem, Adma Soraia Serea
Seixas, Flávio Augusto Vicente
Barros, Lillian
Fernandes, Ângela
Liberal, Ângela
Ivanov, Marija
Soković, Marina
Linde, Giani Andrea
Colauto, Nelson Barros
do Valle, Juliana Silveira
Document Type:
Article (Published version)
,
© 2022, The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology
Metadata
Show full item recordAbstract:
The mycelial biomass of basidiomycetes is a promising source of compounds and represents an alternative for industrial and biotechnological applications. Fungi use light as information and hold photoresponse mechanisms, in which sensors respond to light wavelengths and regulate various biological processes. Therefore, this study aimed to investigate the effects of blue, green, and red lights on the growth, chemical composition, and antioxidant and antimicrobial activity of Lentinus crinitus mycelial biomass. The chemical composition of the mycelial biomass was determined by chromatographic methods, antioxidant activity was analyzed by in vitro assays, and antimicrobial activity was investigated by the microdilution assay. The highest mycelial biomass yield was observed under blue-light cultivation. Many primordia arose under blue or green light, whereas the stroma was formed under red light. The presence of light altered the primary fungal metabolism, increasing the carbohydrate, tocopherol, fatty acid, and soluble sugar contents, mostly mannitol, and reducing the protein and organic acid concentrations. Cultivation under red light increased the phenol concentration. In contrast, cultivation under blue and green lights decreased phenol concentration. Benzoic and gallic acids were the main phenolic acids in the hydroalcoholic extracts, and the latter acids increased in all cultures under light, especially red light. Mycelial biomass cultivated under red light showed the highest antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ferric reducing antioxidant power (FRAP) method showed that all light wavelengths increased the antioxidant activity of mycelial biomass, with the highest value under red light. Moreover, the beta-carotene/linoleic acid co-oxidation (BCLA) assay demonstrated that the antioxidant activity was affected by light cultivation. Mycelial biomass grown under all conditions exhibited antibacterial and antifungal activities. Thus, mycelial biomass cultivation of L. crinitus under light conditions may be a promising strategy for controlling the mycelial chemical composition and biomass yield.
Keywords:
Antioxidant activity; Basidiomycete; LED; Mycelial biomass yield; Phenolic composition; Visible lightSource:
Photochemical & Photobiological Sciences, 2022Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Universidade Paranaense (36900/2020)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brazil (CAPES)
- Fundacao Araucaria
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, 307953/2017-3)
- Foundation for Science and Technology (FCT, Portugal)
DOI: 10.1007/s43630-022-00344-7
ISSN: 1474-905X
PubMed: 36417143
WoS: 000886886100001
Scopus: 2-s2.0-85142460139
URI
https://link.springer.com/10.1007/s43630-022-00344-7http://radar.ibiss.bg.ac.rs/handle/123456789/5338