Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction
2022
Authors:
Gopčević, KristinaGrujić, Slavica
Arsenijević, Jelena
Džamić, Ana
Veličković, Ivona
Izrael-Živković, Lidija
Medić, Ana
Mudrić, Jelena
Soković, Marina
Đurić, Ana
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
The aim of the study was to investigate the biological activity and chemical composition of Satureja kitaibelii Wierzb. ex Heuff. LC–PDA/MS analyses for the aqueous extracts (A1-stem, leaves and flowers, A2-leaves and flowers) and ethyl-acetate extracts (E1-stem, leaves and flowers, E2-leaves and flowers) obtained by ultrasound-assisted extraction enabled the identification of thirty-four compounds. Quantitative analysis revealed that the aqueous extract obtained from leaves and flowers was the richest in total phenolic acids (65.36 mg/g) and flavonoids (21.17 mg/g). The total polyphenol content was the highest in the aqueous extract obtained from leaves and flowers (274 ± 2.4 mg Gallic Acid equivalents/g). The best antioxidant activity was observed for the same extract using the DPPH (SC50 20 ± 10 µg/mL), ABTS (2.834 ± 0.02 mg Ascorbic Acid/g), FRAP (1.922 ± 0.03 mmol Fe 2+ /mg), and total reducing power tests (16.4 ± 1.0 mg Ascorbic Acid/g). Both ethyl acetate extracts were the most active against strains of Bacillus cereus and Micrococcus flavus (MIC 1.70–1.99 mg/mL and 1.99–3.41 mg/mL, respectively). They were more efficient against Aspergillus ochraceus (MFC 0.86 mg/mL) and towards HeLa cell lines. All the obtained results implied the good potential of the investigated extracts to be used as effective preservatives and functional ingredients in food products and dietary supplements.
Source:
Scientific Reports, 2022, 12, 1, 21221-Funding / projects:
- Micromorphological, phytochemical and molecular investigations of plants - systematic, ecological and applicative aspects (RS-MESTD-Basic Research (BR or ON)-173029)
- Molecular, biochemical and immunological tumor diagnosis (RS-MESTD-Basic Research (BR or ON)-175056)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200161 (University of Belgrade, Faculty of Pharmacy) (RS-MESTD-inst-2020-200161)
DOI: 10.1038/s41598-022-25668-3
ISSN: 2045-2322
PubMed: 36481842
Scopus: 2-s2.0-85143592160
URI
https://www.nature.com/articles/s41598-022-25668-3http://radar.ibiss.bg.ac.rs/handle/123456789/5349