Enhanced metabolism of gibberellins is involved in the induction of somatic embryogenesis from root apices of spinach
2022
Аутори:
Belić, MajaTarkowská, Danuše
Zdravković-Korać, Snežana
Milojević, Jelena
Остала ауторства
Milutinović, MilicaТип документа:
Конференцијски прилог (Објављена верзија)
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© 2022 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Gibberellic acid (GA3) and α-naphthaleneacetic acid (NAA) are essential for efficient somatic embryo (SE) induction from root apices of spinach seedlings, while neither is sufficient to provoke an embryogenic response. To gain insight into the role of gibberellins (GAs) in SE induction, the levels of bioactive GAs, their precursors and inactivation products, were determined in embryogenic and non-embryogenic explants, cultivated on medium supplemented with 20 μM NAA + 5 μM GA3 and 20 μM NAA, respectively, for 24 h and 7 days, and in control explants - root apices that were frozen immediately after isolation. The content of GAs was determined using ultra-high performance liquid chromatography coupled to tandem mass spectrometry. The levels of biosynthetic GA precursors GA44, GA19 and GA20 were 7–18-fold higher in embryogenic explants, and only 1.1–2.8-fold higher in non-embryogenic explants compared to the control. In embryogenic explants, the levels of bioactive GA1, GA3, GA4 and GA7 increased 843-, 50433-, 60- and 136-fold, respectively, after 24 h, and remained high after 7 days of treatment. In non-embryogenic explants, only a transient increase of GA3 level (22-fold) was observed after 24 h of treatment. Expectedly, the products of GA2ox inactivation (especially GA8) were significantly higher in embryogenic than in non-embryogenic and control explants. The results evidence a positive impact of enhanced GA metabolism on the acquisition of embryogenic competence, confirming their important role in SE induction and indicating that the 13 hydroxylation pathway is a prevalent pathway of bioactive GA synthesis in embryogenic explants.
Кључне речи:
Gibberellins; Root apices; Spinacia oleracea; Somatic embryogenesisФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- European Regional Developmental Fund Project ‘Centre for Experimental Plant Biology’ No. CZ.0 2.1.01/0.0/0.0/16_019/0000738
У:
- Milutinović M, editor. Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 26.