Changing the plant tissue morphology and physiology by plasma treatment
2021
Autori:
Puač, NevenaŠkoro, Nikola
Živković, Suzana
Milutinović, Milica
Jevremović, Slađana
Petrović, Zoran Lj.
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
,
© 2021 by the Leibniz Institute for Plasma Science and Technology
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
In parallel with the plethora of biomedical applications that employ atmospheric pressure plasma systems, another field of plasma applications is growing – plasma agriculture [1]. Chemical species in plasma, with ample amounts of Reactive Oxygen and Nitrogen Species (RONS), are responsible for triggering various mechanisms and effects in plant cells. For example in treatment of seeds, the rich plasma chemistry changes the coat of the treated seed resulting in changes of wettability, better water uptake, an increased percentage and speed of germination [1-3]. Another application of atmospheric pressure plasmas is for treatments of plant calli In biological research and biotechnology the plant callus (pl. calli) is induced from plant tissue and it forms growing mass of plant meristematic cells. Plant callus is widely used in plant biology both for basic research and industrial production: in plant biotechnology
as a tool for genetic manipulation of plants, for micropropagation, for studies of plant metabolism and cellular development, commercial production of natural products that cannot be chemically synthesized etc.. Puač et al. have studied the influence of RONS on Daucus carota calli showing their long-term influence [4]. We have used plasma needle type of the atmospheric pressure plasma device for direct plasmas treatments of
plant calli. The operational frequency of the device was 13.56 MHz and working gas was helium. The flow of
helium was kept constant at 1 slm The detailed characterization of the discharge was performed by optical emission
spectroscopy, mass spectroscopy and electrical measurements The direct plasma treatment of the plant calli was
used to investigate the plasma-cell interactions and to follow the response of the plant tissue several hours and days
after the treatment We have used calli of model plant (Daucus carota) and of plants with specific issues, like small
bearded irise (Iris reichenbachii) who mainly produce non regenerative calli that do not enter the process of somatic
embryogenesis (SE) Firstly, in treatments of D.carota calli we found that the formation of SE can be initiated even
under non- permissive conditions. Similar effects were obtained with I. reichenbachii calli where the atmospheric
plasma treatment induced significant morphological and physiological changes in non-embryonic calli toward SE
formation that were followed also with the enhanced production of arabinogalactan proteins
Finansiranje / projekti:
- Primene niskotemperaturnih plazmi u biomedicini, zaštiti čovekove okoline i nanotehnologijama (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41011)
- Fundamentalni procesi i primene transporta čestica u neravnotežnim plazmama, trapovima i nanostrukturama (RS-MESTD-Basic Research (BR or ON)-171037)
- Biotehnologija in vitro - gajene, lekovite i ugrožene biljne vrste (RS-MESTD-Basic Research (BR or ON)-173015)
- Razvoj i primena biotehnoloških postupaka u dobijanju zdravog sadnog materijala ukrasnih biljaka (RS-MESTD-Technological Development (TD or TR)-31019)
U:
- Book of Abstracts: 3rd International Workshop on Plasma Agriculture: IWOPA2021; 2021 Mar 1-3; Greifswald, Germany. Leibniz Institute for Plasma Science and Technology; 2021. p. 14.