dc.creator | Jovanović Stojanov, Sofija | |
dc.creator | Stepanović, Ana | |
dc.creator | Ljujić, Mila | |
dc.creator | Lupšić, Ema | |
dc.creator | Podolski-Renić, Ana | |
dc.creator | Dragoj, Miodrag | |
dc.creator | Jovanović, Mirna | |
dc.creator | Schenone, Silvia | |
dc.creator | Pešić, Milica | |
dc.creator | Dinić, Jelena | |
dc.date.accessioned | 2023-01-31T13:30:08Z | |
dc.date.available | 2023-01-31T13:30:08Z | |
dc.date.issued | 2022 | |
dc.identifier.uri | http://nwm.covr.be/EACR2022abstracts/data/HtmlApp/main.html# | |
dc.identifier.uri | http://radar.ibiss.bg.ac.rs/handle/123456789/5425 | |
dc.description.abstract | Introduction:
Glioblastoma (GBM) is among the most frequent and aggressive brain tumors characterized by
infiltrating nature, high proliferation, and resistance to chemotherapy and radiation. GBM exhibit high
expression of Src tyrosine kinase which regulates proliferation, survival, and invasiveness of tumor
cells, making Src a potential target for GBM therapy. Numerous Src family kinase inhibitors (SFKI)
were reported to induce autophagy, thus protecting cells from undergoing cell death. However,
inhibition of autophagy was shown to sensitize cells to SFKI in several cancer types.
Material and Methods:
Human GBM cell line U87 and its multidrug-resistant (MDR) counterpart U87-TxR were transfected
with RFP-LC3, an autophagy marker. The ability of two SFKIs, pyrazolo[3,4-d]pyrimidines Si306 and
its prodrug pro-si306, to induce autophagy in RFP-LC3-transfected GBM cells was evaluated by flow
cytometry and fluorescent microscopy. Cell viability was assessed by MTT assay. The autophagy
induction and autophagic flux were evaluated by Acridine orange assay, immunocytochemistry and
immunoblotting. Cell proliferation rate was analyzed by CFSE assay. Cell death was detected by
Annexin/Propidium Iodide assay. PARP-1 cleavage was assessed by immunoblotting.
Results and Discussions:
SFKI treatment resulted in degradation of RFP-LC3 after 3 h treatment as well as in formation of
RFP-LC3 puncta in GBM cells demonstrating autophagy induction. The effect of SFKIs on autophagy
induction persisted after 48 h, as demonstrated by autophagy markers LC3 and p62. Inhibition of
autophagy by Bafilomycin A1 sensitized both U87 and U87-TxR cells to Si306 and its pro-drug after
48 h. The anti-proliferative effect of Si306 and pro-Si306 was additionally increased after autophagy
inhibition by Bafilomycin A1. Furthermore, while single SFKI treatments did not cause significant
cell death, combination treatments with autophagy inhibitor induced necrosis in U87 and U87-TxR
cells after 48 h. Detection of necrotic PARP-1 fragment further confirmed necrotic cell death.
Conclusion:
Taken together, these data suggest that autophagy induced by Si306 and pro-Si306 has a protective
role in GBM cells, and that autophagy modulation may be used to enhance the anticancer effects of
SFKIs. In addition, as the ability of the SFKIs to induce autophagy was not diminished by the
presence of the MDR phenotype makes these compounds promising for treatment of MDR cancers. | sr |
dc.language.iso | en | sr |
dc.publisher | European Association for Cancer Research | sr |
dc.rights | openAccess | sr |
dc.source | Congress abstracts: Annual Congress of the European Association for Cancer Research EACR 2022: Innovative Cancer Service: Translating Biology to Medicine; 2022 Jun 20-23; Seville, Spain | sr |
dc.title | Autophagy inhibition sensitises glioblastoma cells to Src family kinase inhibitors Si306 and its prodrug | sr |
dc.type | conferenceObject | sr |
dc.rights.license | ARR | sr |
dc.rights.holder | © 2022 by the European Association for Cancer Research | sr |
dc.description.other | Congress abstracts: Annual Congress of the European Association for Cancer Research EACR 2022: Innovative Cancer Service: Translating Biology to Medicine; 2022 Jun 20-23; Seville, Spain. European Association for Cancer Research; 2022. p. P1-135. | sr |
dc.citation.spage | P1-135 | |
dc.type.version | publishedVersion | sr |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/12229/bitstream_12229.pdf | |
dc.citation.rank | M34 | |
dc.citation.rank | M34 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_5425 | |