Somatic embryogenesis of Centaurium erythraea Rafn. time-lapse documentation of in vitro development
2018
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Authors:
Bogdanović, MilicaĆuković, Katarina
Dragićević, Milan
Simonović, Ana
Todorović, Slađana
Contributors
Kovačević, DušanDocument Type:
Conference object (Published version)
,
© 2018 by the Faculty of Agriculture, University of East Sarajevo
Metadata
Show full item recordAbstract:
Organogenesis and somatic embryogenesis (SE) are often used for mass propagation
of high quality material of medicinal, endangered and rare plant species. In Centaurium
erythraea Rafn. (Gentianaceae), used as a model system in developmental studies, both
pathways can be exploited. SE from centaury leaf tissues starts with the formation of
embryogenic callus which develops into somatic embryos following globular, heart, torpedo and cotiledonary embryo phases. SE potential of leaf explants and embryogenic calli is highly dependent on concentration and ratio of added plant growth regulators, genotype, explant type and number of subcultures. Frequent (weekly) subculturing of the calli slows down growth and differentiation, whereas biweekly subculturing results in better embryogenic response. Higher CPPU to 2,4-D ratio drives the callus development towards embryo differentiation. Embryogenic potential of the callus cultures also depends on the presence of already formed globular embryos, since their removal reduces both growth and embryogenic potential. Once this potential is reduced, it cannot be restored by increasing exogenous hormone concentration. In order to further characterize and improve these processes in centaury, a documentation system was developed, using LED white light epi illumination, coupled with a smartphone camera with macro lens. Developmental processes were observed sequentially on leaf sections subjected to different 2,4-D and CPPU concentrations. Image processing of focal
stacks from developing explants was automated in Adobe Photoshop and Bridge. A relational database containing all relevant sample information and photographs was then built using Excel and R.
Keywords:
development; somatic embryogenesis; 2,4-D; CPPUFunding / projects:
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
In:
- Kovačević D, editor. Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina. East Sarajevo: Faculty of Agriculture; 2018. p. 347.