Tissue specific expression and genomic organization of sesquiterpene lactone biosynthetic genes in chicory
2016
Аутори:
Cankar, KatarinaBogdanović, Milica
Todorović, Slađana
Dragićević, Milan
Simonović, Ana
van Houwelingen, Adele
Schijlen, Elio
van de Geest, Henri
Hendriks, Theo
Quillet, MC
Bouwmeester, Harro
Bosch, Dirk
Beekwilder, Jules
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2016 by the Phytochemical Society of Europe
Метаподаци
Приказ свих података о документуАпстракт:
Sesquiterpene lactones (STLs) give chicory its characteristic bitter taste and are highly concentrated in the latex channels of chicory taproot. The study of STLs is interesting to control the bitterness of industrial and consumption chicory, including witloof. The major STLs in chicory are lactucin, deoxylactucin, and lactucopicrin and oxalate and sulphate conjugates thereof. Formation of chicory STLs requires the biosynthesis of costunolide. Two germacrene A synthases, two germacrene A oxidases and a costunolide synthase have been described from chicory. In this work, the genetic organization of the genes involved in STL biosynthesis in chicory linkage groups is described. A number of genomic regions hosting STL biosynthetic genes have been addressed by NGS sequencing. Gene expression in different tissues is addressed by following their expression
using an RNAseq approach and by promoter activity studies using GFP. Finally, the downregulation of STL gene expression in chicory is achieved in hairy root culture regenerants expressing amiRNAs, and the consequences for STL content in different tissues is tested. These results will contribute to successful breeding strategies for chicory germplasm with well-defined STL content and bitterness.
Кључне речи:
sesquiterpene lactones; chicory; NGS sequencing; amiRNA silencing; GFP; promoterФинансирање / пројекти:
У:
- Book of abstracts: Plant omics and biotechnology for human health: POBHH; 2016 Nov 21-24; Gent, Belgium. Ghent : Phytochemical Society of Europe; 2016. p. 64.