Effects of macrophage migration inhibitory factor deficiency and fructose-enriched diet on systemic and hippocampal insulin sesitivity, behavior and hippocampal plasticity in male mice
2017
Аутори:
Gligorovska, LjupkaĐorđević, Ana
Bursać, Biljana
Veličković, Nataša
Ignjatović, Đurđica
Tomić, Mirko
Matić, Gordana
Остала ауторства
Kanazir, SelmaBjelobaba, Ivana
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2017 by the Serbian Neuroscience Society
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Приказ свих података о документуАпстракт:
Introduction: The macrophage migration inhibitory factor (MIF) is involved in the regulation of glucose metabolism in the hippocampus, while fructose overconsumption has been associated with changes in hippocampal insulin sensitivity and synaptic plasticity, resulting in disturbances in learning and memory. Therefore, we hypothesized that MIF defficiency in combination with fructose diet (FD) may affect insulin sensitivity and hippocampal synaptic plasticity, thus leading to behavioral changes.
Methods: The effects of 9-week 20% FD on energy intake and insulin sensitivity in wild type (WT) and MIF deficient (MIF−/−) C57Bl/6J male mice were analyzed. Inhibitory Ser307 phosphorylation of insulin receptor substrate 1 (IRS-1) was used as hallmark of hippocampal insulin resistance. Novel object recognition test (NOR) was used to assess exploratory behavior. Synaptic plasticity was estimated by polysialylated-neural cell adhesion molecule (PSA-NCAM) protein level.
Results: WT and MIF−/− mice on fructose diet had increased energy intake, while systemic insulin sensitivity was disturbed in all MIF−/− mice. Hippocampal pIRS-1 Ser307 was elevated in all animals compared to WT on standard diet (SD). MIF−/− animals on SD showed impaired recognition memory in NOR. Finally, although PSA-NCAM was decreased in WT animals on FD and MIF−/− on SD, its level in fructose-fed MIF−/− mice was the same as in the WT on SD.
Conclusion: These preliminary results show that both MIF defficiency and fructose caloric overload are implicated in impairment of systemic and hippocampal insulin sensitivity. However, behavioral changes observed in MIF−/− mice were normalized after fructose feeding, which coincided with the changes in synaptosomal PSA-NCAM protein level.
Финансирање / пројекти:
- Улога стероидних хормона у неуроендокриној адаптацији на стрес и патофизиологији метаболичког синдрома - молекуларни механизми и клиничке импликације (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
У:
- Kanazir S, Bjelobaba I, editors. Book of Abstract: 7th Congress of Serbian Neuroscience Society with international participation; 2017 Oct 25-27; Belgrade, Serbia. Belgrade: Serbian Neuroscience Society; 2017. p. 97.