Secoiridoid glycosides production in hairy roots of Gentiana pneumonanthe L.: optimization of cytokinin concentration in the culture medium
2015
Аутори:
Božić, DraganaSkorić, Marijana
Šiler, Branislav
Živković, Suzana
Todorović, Slađana
Dmitrović, Slavica
Banjanac, Tijana
Mišić, Danijela
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Gentiana pneumonanthe L. (fam. Gentianaceae) is a herbaceous perennial growing on wet
heathlands, grasslands and hay meadows of Europe and Asia. Like other Gentiana species, G.
pneumonanthe is characterized by the production of bitter secoiridoid glycosides (SGs) widely
used in the pharmaceutical industry. Hairy roots (HRs) of G. pneumonanthe, as an alternative
source of these phytochemicals, have been induced by agropine type Agrobacterium rhizogenes
strain (A40M70GUS), and were characterized by genetic stability, sizable biomass production, and
increased biosynthetic capacity compared to the native plant roots and above ground parts. Although
HRs of G. pneumonanthe showed intensive growth without the application of phytohormones, the
effect of varying concentrations of cytokinins (BAP – benzylaminopurine; KIN – kinetin) on the
morphogenesis and on the content of SGs has been investigated in the present study, with the
aim of optimizing in vitro conditions for enhanced production of targeted secondary metabolites.
Morphogenetic changes, such as the formation of calli, were induced by both phytohormones.
Significant reduction of HR biomass occurred only on treatments with high BAP and KIN
concentrations (3 and 4 mg l-1). The content of SGs, especially gentiopicrin and sweroside, was
affected by both KIN and BAP. The lowest applied concentration of these phytohormones (0.1 mg
l-1) showed a stimulatory effect on the production of gentiopicrin and sweroside. Further increase
in both cytokinins concentrations decreased the content of these SGs.
Being genetically stable and fast-growing, HR cultures of G. pneumonanthe could provide a constant
and highly productive source of valuable secondary metabolites, and could further be implemented
in large-scale bioreactors. Although many challenges still exist for the commercial implementation
of this alternative production system, the results presented here demonstrate the opportunities for
enhanced SGs production by the application of in low concentrations of both cytokinins and thus
highlight the benefits of optimizing growth conditions.
Кључне речи:
Gentiana pneumonanthe; hairy roots; cytokinins; secoiridoid glycosides; secondary metabolitesИзвор:
Botanica Serbica, 2015, 39, 2, 71-78Финансирање / пројекти:
- Физиолошка, хемијска и молекуларна анализа диверзитета одабраних ретких и угрожених биљних врста у циљу еx ситу заштите и продукције биолошки активних једињења (RS-MESTD-Basic Research (BR or ON)-173024)