Uticaj vodenog ekstrakta ploda aronije na imunski sistem u mišjim modelima infekcije i melanoma
Effects of chokeberry fruit water extract on the immune system in mouse models of infection and melanoma
Abstract:
Chokeberry (Aronia melanocarpa), fruit with black berries that leave an astringent mouthfeel after consumption, is rich in polyphenols responsible for its bioactive properties. Chokeberry extract (VEPA) showed profound in vitro and in vivo pro-inflammatory effects. When applied in vitro, VEPA stimulated phagocytic ability of macrophages and NO production from dendritic cells, as well as differentiation of pro-inflammatory CD4+ T cells. In vivo oral treatment of healthy mice changed the distribution of immune cells within the gut and increased production of IFN-γ in the spleen. In the mouse model of infection with intracellular bacteria Listeria monocytogenes VEPA accelerated eradication of infection and improved their general appearance. Also, VEPA pretreatment increased proportions of macrophages and CD8+ T cells both in the gut and the spleen of infected mice and mainly affected effector functions of macrophages. In the mouse model of melanoma, induced by subcutaneous injection of B16 cells, VEPA delayed melanoma appearance, decreased tumor volume and increased infiltration of immune cells in the tumor. Proportion of NK cells, CD4+ and CD8+ T cells within the tumor was increased, as well as the expression of IFN-γ within these cells, with downregulated frequency of cells that suppress the immune cells. Additionally, the production of IFN-γ was enhanced in the mesenteric lymph nodes, spleen and the serum of pretreated animals. Splenocytes from these animals showed enhanced cytotoxicity towards melanoma cells, dependent on IFN-γ. These results point to the applicative potential of VEPA in the prevention of different states and diseases where an enhanced pro-inflammatory response is needed.
Keywords:
Aronia melanocarpa; Listeria monocytogenes; B16; Chokeberry extract; Intracellular bacteria; Melanoma; Immunomodulation; Pro-inflammatory response; Antibacterial response; Antitumor responseSource:
Faculty of Biology, University of Belgrade, 2023, -110Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-MESTD-Basic Research (BR or ON)-173013)