Astrocyte activity in the central nervous system autoimmunity
2019
Autori:
Nikolić, LjiljanaBijelić, Dunja
Lazarević, Milica
Milićević, Katarina
Momčilović, Miljana
Bogdanović Pristov, Jelena
Petković, Branka
Anđus, Pavle
Miljković, Đorđe
Ostala autorstva
Isaković, AnđelkaTip dokumenta:
Konferencijski prilog (Objavljena verzija)
,
© 2019 by the Serbian Neuroscience Society
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Aims: Multiple sclerosis (MS) is an in ammatory autoimmune disorder of the central nervous system (CNS). Complex
interactions between in ltrating immune cells (IIC) and resident glial cells of the CNS cause myelin loss and neuronal dysfunction
in MS. Here we aim to understand how naïve astrocytes functionally respond to the IIC invasion of the CNS.
Methods: We measured calcium activity of naïve astrocytes in culture upon application of IIC. An experimental autoimmune
encephalomyelitis (EAE) MS rat model was used to isolate IIC from the spinal cord of animals at the symptomatic stage. Naïve
astrocytes were isolated from the spinal cord of WT rats.
Results: We show that IIC and not the lymph node immune cells evoke vigorous increase in the astrocyte calcium activity.
This IIC-induced calcium response depends on an autocrine activation of the purinergic P2X7 receptors on the naïve astrocytes.
We also show that IIC induce ATP release from astrocytes by a mechanism that involves gap junctions and/or hemichannels
activation and not the vesicular pathway. Our data indicate that ATP release and subsequent increase in the astrocytic calcium
activity mainly depends on the cell-cell contact between naïve astrocytes and IIC.
Conclusions: These results show that naïve astrocytes functionally respond to the IIC by augmented release of ATP. An increase
in ATP release would alter astrocyte-neuron communication and a ect neuronal function in MS.
Ključne reči:
Astrocytes; CNS disease; multiple sclerosisU:
- Isaković A, editor. Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. Belgrade: Serbian Neuroscience Society; 2019. p. 295.