Protoplast patch-clamping using an upright microscope with a movable stage
2022
Autori:
Nikolić, LjiljanaVidović, Marija
Todorović, Nataša
Petković, Branka
Stojadinović, Gordana
Martać, Ljiljana
Bogdanović Pristov, Jelena
Ostala autorstva
Čupić, ŽeljkoAnić, Slobodan
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2022 by the Society of Physical Chemists of Serbia
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Investigating membrane properties of plants is a challenging task, considering that success of
experiments is highly dependent on the possibility to isolate metabolically active protoplasts that
can withstand membrane current recordings. The aim of the present work is to obtain viable
protoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patchclamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplasts
with preserved membrane integrity suitable for electrophysiological experiments. We applied a
custom approach for patch-clamping protoplasts using a microscope with a movable microscope
stage. We recorded prominent inward and prominent outward types of membrane current profiles of
protoplasts. Obtained data indicate that optimized isolation protocol and custom system for patchclamping, can be applied to study membrane properties of root protoplasts.
Ključne reči:
plant protoplast; patch-clamp; microscopeFinansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200053 (Univerzitet u Beogradu, Institut za multidisciplinarna istraživanja) (RS-MESTD-inst-2020-200053)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200042 (Univerzitet u Beogradu, Institut za molekularnu genetiku i genetičko inženjerstvo) (RS-MESTD-inst-2020-200042)
U:
- Čupić Ž, Anić S, editors. Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia. Belgrade: Society of Physical Chemists of Serbia; 2022. p. 271-4.