Iron modulates norepinephrine effect on astrocytes
2019
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2019 by the COST Action CA15133
Metapodaci
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Iron, an essential element for living organisms, participates in a wide range of metabolic
processes. It appears predominantly firmly bound to proteins, but can also be loosely bound to
low-affinity ligands, referred as labile iron pool (LIP). The composition and amount of LIP can
vary considerably under different physiological conditions, playing a beneficial role in iron
economy and homeostasis or contributing to the generation of reactive oxygen species. It is
still not known if bioactivity of low-affinity ligands can be modulated by iron binding.
Catecholamine neurotransmitters including norepinephrine (NE) can chelate iron. In the close
vicinity of synaptic cleft, astrocytes are direct target of norepinephrine. Here we show on
cultured rat cortical astrocytes that iron bound to NE completely blocks neurotransmitter
activity of NE. However, how astrocyte activity changes when norepinephrine binds iron
remains unknown.
We show, using spectrophotometry that NE and Fe3+ form complex in the 1:1 stoichiometry,
at pH 7.4. Iron effect on astrocyte response to NE was examined by the whole-cell patch-clamp
technique. NE alone evokes changes in the membrane currents of astrocytes, but such effects
were not observed for the NE- Fe3+ complex.
Our results demonstrating that iron in the complex with norepinephrine inhibits alpha-adrenergic
receptors and modulates astrocyte activity, imply a novel neuromodulatory role for LIP.
Ključne reči:
norepinephrine; iron; astrocyteU:
- Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland. COST Action CA15133; 2019.