Negative features of some dental products containing eucalyptol: Upregulation of genes expression encoding for efflux pumps in Candida albicans
2022
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2022 by the Matica srpska
Метаподаци
Приказ свих података о документуАпстракт:
Eucalyptol is the dominant compound of eucalyptus oil and an ingredient of many products
used for teeth whitening and washing. This study aimed to reveal the influence of eucalyptol
on Candida albicans growth isolated from oral cavity and to test expression of virulence
traits in the presence of this compound. In this study, we examined the ability of eucalyptol to
inhibit the growth of different Candida strains in suspension and biofilm, to block hyphal
transition along with their impact on genes encoding for efflux pumps (CDR1 and CDR2) and
ergosterol biosynthesis (ERG11). In the case of patients suffering from fungal infections,
upregulation of CDR1 and CDR2 is an undesirable property for any therapeutic given along
the way, while their downregulation is seen as a promising antifungal trait. Eucalyptol
showed antifungal activity with a minimal inhibitory concentration of 2–23 mg/mL. The
application of eucalyptol reduced the formation of biofilm biomass by more than 50% in
three C. albicans strains (C. albicans ATCC 10231, C. albicans 475/15 and C. albicans
503/15) at their MIC concentrations. The application of eucalyptol (23 mg/mL) induced a
notable reduction in the number of hyphal cells. Treatment with eucalyptol led to the
increased expression of CDR1 and CDR2 and did not interfere with the ERG11 expression.
Although, eucalyptol showed some antifungal activity against Candida strains, its use in
cosmeceutical dental products should be with caution. Patients having problems with oral
Candida infections and using antifungal drugs should not use preparations based on
eucalyptol since the compound showed negative features on expression of genes encoding for
efflux pumps, possibly leading to lower effectiveness of antifungal drugs.
Кључне речи:
eucalyptol; Candida albicans; virulence traits; dental products; efflux pumpsФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia. Novi Sad: Matica srpska; 2022. p. 35.