Establishment of association of an Mg2+-dependent endonuclease with the rat liver nuclear matrix in cryonecrosis
2007
Аутори:
Grdović, NevenaMihailović, Mirjana
Vidaković, Melita
Dinić, Svetlana
Uskoković, Aleksandra
Martinović, Vesna
Arambašić Jovanović, Jelena
Grigorov, Ilijana
Ivanović-Matić, Svetlana
Bogojević, Desanka
Petrović, Miodrag
Poznanović, Goran
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2007 John Wiley & Sons, Ltd.
Метаподаци
Приказ свих података о документуАпстракт:
Previously, we characterized the endonucleolytic activity of the nuclear matrix prepared from rat liver cryopreserved in liquid
nitrogen. The enzymic activity was attributed to a 23 kDa, Mg2þ
-dependent and sequence non-specific endonuclease (p23)
stably associated with the nuclear matrix. Here we show that p23 was absent from the nuclear matrix prepared from fresh
liver. Instead, both ex vivo (cryopreservation), as well as in vivo-induced necrosis by repeated freezing/thawing of liver tissue
in an anaesthetized rat, promoted the activation and translocation of p23 to the nuclear matrix. Considering that ex vivo and
in vivo freezing/thawing of the liver were accompanied by morphological (nuclear compaction) and biochemical events
(increased LDH activity, disorderly genomic DNA degradation, absence of lamin proteolysis, appearance of 62 and 50 kDa
necrotic cleavage products of PARP-1) commonly observed during necrosis, and because the association of p23 with the
nuclear matrix was saturable, reflecting the existence of a limited number of distinct high affinity sites on the nuclear matrix
for p23, we concluded that the activation of the nuclear matrix-associated endonuclease p23 is a feature of liver cryonecrosis.
Although cryonecrosis represents a typical example of acute cell damage, our results suggest that it is realized by ordered
molecular events.
Кључне речи:
cryonecrosis; Mg2þ -dependent endonuclease; nuclear matrixИзвор:
Cell Biochemistry and Function, 2007, 25, 3, 345-355Финансирање / пројекти:
- Акутни и хронични стрес: механизми регулације хомеостазе у акутној радијационој болести и дијабетесу (RS-MESTD-MPN2006-2010-143002)
DOI: 10.1002/cbf.1407
ISSN: 0263-6484
PubMed: 17410538