Hippocampal sleep spindle dynamics during REM sleep and their distinct underlying parvalbumin and synaptic proteins expression in the reticulo-thalamic nucleus of the parkinsonian rats
2022
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2022 by the Federation of European Neuroscience Societies
Метаподаци
Приказ свих података о документуАпстракт:
We investigated the alterations of reticulo-thalamic (RT) GABAergic parvalbumin (PV+) interneurons and synaptic reorganization underlying the altered hippocampal high voltage sleep spindle (HVS) dynamics during REM sleep in the rat
models of Parkinson’s disease (PD). Adult male Wistar rats were implanted for 6h sleep recording during light phase in four
experimental groups: control (implanted controls), PD cholinopathy (bilateral lesion of the nucleus pedunculopontinus
tegmentalis−PPT), hemiparkinsonism (unilateral lesion of the nucleus substantiae nigrae pars compacta−SNpc) and
hemiparkinsonism with PD cholinopathy (unilateral SNpc/bilateral PPT lesion). Following 14 days of the surgical procedure
we differentiated the Wake/NREM/REM 10s epochs, and the HVSs detection and extraction was done automatically (4.1–10
Hz band pass filter, 1s minimum duration) and visually validated. Hippocampal HVS dynamics were analyzed during 1h of
NREM/REM sleep. Alterations of the PV+ interneurons and synaptic re-organization within the RT were determined by the
parvalbumin, MAP2 and PSD-95 immunostaining. REM sleep is a predisposing state for the HVSs induction in all
experimental models of PD neuropathology. Whereas the PD cholinopathy induced the prolongation and higher density of
hippocampal HVSs, the hemiparkinsonism with PD cholinopathy increased the hippocampal HVSs intrinsic frequency during
REM sleep. In contrast to the unaltered PV+ interneurons/partially enhanced MAP2/suppressed PSD-95 expression during
PD cholinopathy, we evidenced the PV+ interneurons reduction/enhanced MAP2/no change of PSD-95 expression in the RT
during hemiparkinsonism with PD cholinopathy. Distinct PV+ interneurons alteration and inhibition/excitation balance in the
RT could be the underlying mechanisms of HVS generation/alteration during REM sleep in the parkinsonian rats.
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France. Federation of European Neuroscience Societies; 2022. p. 2231-2.