Physiological and antioxidant defense mechanisms of Nepeta nervosa plants against polyethylene glycol (PEG)-mediated drought stress
2022
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Authors:
Dmitrović, SlavicaNestorović Živković, Jasmina
Aničić, Neda
Skorić, Marijana
Gašić, Uroš
Milutinović, Milica
Matekalo, Dragana
Petrović, Luka
Božunović, Jelena
Filipović, Biljana
Banjanac, Tijana
Šiler, Branislav
Todorović, Miloš
Lukić, Tamara
Mišić, Danijela
Contributors
Milutinović, MilicaDocument Type:
Conference object (Published version)
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© 2022 by the Serbian Plant Physiology Society
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Show full item recordAbstract:
Drough-stress conditions lead to excessive production of reactive oxygen species (ROS) and
oxidative damage in plants. Plants display various defense mechanisms for survival, including the
activation of antioxidant enzymes and the production of low molecular weight non-enzymatic
antioxidants like phenolics. In this work, roots of four weeks old Nepeta nervosa plants were exposed to PEG solutions with osmotic pressure of −3 MPa for one, three and six days. Fresh weight
(FW) and relative water content (RWC) of N. nervosa plants were significantly reduced compared
to the corresponding control - up to 68% (FW) and up to 35% (RWC). In leaves, the content of rosmarinic and chlorogenic acids, dominant phenolic compounds, as well as of less abundant ferulic
and caffeic acids, was dependent on the duration of PEG-induced drought stress. The content of
rosmarinic acid in control plants achieved an average value of 18.27 µg/mg FW, while at PEG-treatments it increased by 355% (3 days of PEG-treatment). Among all examined antioxidative enzymes
in leaves of PEG treated N. nervosa plants, superoxide dismutases (SOD) were the most active (an
increase of 853% on day 6). PEG treatment also caused an increase in peroxidase (POX) activity in
leaves of N. nervosa plants (108% increase on day 3). Since catalase (CAT) activity was significantly decreased on PEG treatment, except on day 1, it could be presumed that H2O2 elimination is
performed mostly by POX. This work is a useful contribution to the development of a strategy for
overcoming oxidative stress in Nepeta species, especially in arid and semiarid regions.
Keywords:
water stress; antioxidative enzimes; Nepeta nervosa; phenolic acidFunding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- NEPETOME - Omics-guided disentangling of the iridoid diversity in the genus Nepeta L. for in planta metabolic engineering towards sustainable exploitation (RS-ScienceFundRS-Ideje-7749433)
In:
- Milutinović M, editor. Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 68.